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Tytuł:
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An efficient fluorescence sensor for nitroreductase selective imaging based on intramolecular photoinduced electron transfer.
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Autorzy:
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Zhang N; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; School of Life and Environment Sciences, Harbin University of Commerce, Harbin 150076, China.
Wang Y; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; School of Life and Environment Sciences, Harbin University of Commerce, Harbin 150076, China.
Leng S; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Xu S; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhang L; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Wang Q; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhang Q; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address: .
Hu HY; State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. Electronic address: .
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Źródło:
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Talanta [Talanta] 2019 Dec 01; Vol. 205, pp. 120133. Date of Electronic Publication: 2019 Jul 09.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
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MeSH Terms:
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Optical Imaging*
Photochemical Processes*
Nitroreductases/*metabolism
Spectrometry, Fluorescence/*instrumentation
Carbocyanines/chemistry ; Electron Transport ; Hydrogen-Ion Concentration ; Methicillin-Resistant Staphylococcus aureus/metabolism ; Nitroreductases/antagonists & inhibitors ; Temperature
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Contributed Indexing:
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Keywords: Bacteria; Cyanine; Fluorescence imaging; Nitroreductase; Photoinduced electron transfer
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Substance Nomenclature:
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0 (Carbocyanines)
0 (cyanine dye 5)
EC 1.7.- (Nitroreductases)
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Entry Date(s):
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Date Created: 20190828 Date Completed: 20200203 Latest Revision: 20200203
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Update Code:
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20240105
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DOI:
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10.1016/j.talanta.2019.120133
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PMID:
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31450408
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Timely and effective detection of bacterial pathogens is of great importance to reduce morbidity rates from bacterial infections. Recently, enzyme-activated fluorogenic probes, which invoke enzymatic catalysis to trigger fluorescence emission, have been superior sensors for bacterial infections needed for accurate diagnoses. Here, a fluorescent sensor for nitroreductase (NTR) detection is described. It is based on a cyanine fluorophore and utilizes photoinduced electron transfer to generate a rapid 10-fold fluorescence response after being catalytically reduced by NTR. It has enabled selective and sensitive visualization of NTR activity in vitro and in living bacterial pathogens. Thus, the probe has great potential to provide a rapid, noninvasive tool to diagnose infections and guide antimicrobial selection.
(Copyright © 2019 Elsevier B.V. All rights reserved.)