Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity.

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Abstrakt

Tytuł:: Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity.
Autorzy:: Scott EM; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK.
Frost S; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK.
Khalique H; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK.
Freedman JD; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK.
Seymour LW; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK.
Lei-Rossmann J; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK. .
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2058, pp. 261-270.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Ascites*
Ascitic Fluid*
Genetic Vectors*/genetics
Liquid Biopsy*/methods
Oncolytic Virotherapy*/methods
Oncolytic Viruses*/genetics
Adenoviridae/genetics ; Biomarkers ; Epithelial Cell Adhesion Molecule/genetics ; Epithelial Cell Adhesion Molecule/metabolism ; Humans ; Immunophenotyping ; Neoplasms/diagnosis ; Neoplasms/therapy
Grant Information:: 29106 United Kingdom CRUK_ Cancer Research UK; C5255/A20936 United Kingdom CRUK_ Cancer Research UK; C552/A17720 United Kingdom CRUK_ Cancer Research UK; MR/N013468/1 United Kingdom MRC_ Medical Research Council
Contributed Indexing:: Keywords: Ascites; Ex vivo model; Flow cytometry; Liquid biopsies; Oncolytic virus
Substance Nomenclature:: 0 (Biomarkers)
0 (EPCAM protein, human)
0 (Epithelial Cell Adhesion Molecule)
Entry Date(s):: Date Created: 20190906 Date Completed: 20201214 Latest Revision: 20240210
Update Code:: 20240210
DOI:: 10.1007/978-1-4939-9794-7_17
PMID:: 31486044
: Czasopismo naukowe

The translational success of oncolytic virotherapies would benefit from the widespread use of clinically relevant ex vivo models. Malignant ascites, an accumulation of fluid in the peritoneum due to disseminated cancer, recapitulates many features of the tumor microenvironment, making it a valuable model for studying oncolytic virus activity. Here, we describe a method for the separation and storage of cellular and acellular components of malignant ascites, followed by flow cytometric characterization of the cellular fraction. We then outline a simple experiment using whole ascites to assess the activity of a bispecific T cell engager (BiTE)-expressing oncolytic adenovirus.

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