Ultra-thin sectioning and grinding of epoxy plastinated tissue.

Tytuł:: Ultra-thin sectioning and grinding of epoxy plastinated tissue.
Autorzy:: Sora MC; Centre for Anatomy and Molecular Medicine, Sigmund Freud University Vienna, Vienna, Austria.
von Horst C; HC Biovision, Mainburg, Germany.
López-Albors O; Department Anatomy and Comparative Pathological Anatomy, University of Murcia, Murcia, Spain.
Latorre R; Department Anatomy and Comparative Pathological Anatomy, University of Murcia, Murcia, Spain.
Źródło:: Anatomia, histologia, embryologia [Anat Histol Embryol] 2019 Nov; Vol. 48 (6), pp. 564-571. Date of Electronic Publication: 2019 Sep 05.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Berlin : Wiley-Blackwell
Original Publication: Berlin, Parey.
MeSH Terms:: Anatomy/*methods
Microtomy/*methods
Plastination/*methods
Animals ; Epoxy Resins ; Humans ; Microtomy/instrumentation ; Staining and Labeling/methods
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Contributed Indexing:: Keywords: education; plastination; sectional anatomy
Substance Nomenclature:: 0 (Epoxy Resins)
Entry Date(s):: Date Created: 20190906 Date Completed: 20200323 Latest Revision: 20200323
Update Code:: 20240105
DOI:: 10.1111/ahe.12478
PMID:: 31487077
: Czasopismo naukowe

Pełny tekst

With classical sheet plastination techniques such as E12, the level and thickness of the freeze-cut sections decide on what is visible in the final sheet plastinated sections. However, there are other plastination techniques available where we can look for specific anatomical structures through the thickness of the tissue. These techniques include sectioning and grinding of plastinated tissue blocks or thick slices. The ultra-thin E12 technique, unlike the classic E12 technique, starts with the plastination of a large tissue block. High temperatures (30-60°C) facilitate the vacuum-forced impregnation by decreasing the viscosity of the E12 and increasing the vapour pressure of the intermediary solvent. By sectioning the cured tissue block with a diamond band saw plastinated sections with a thickness of <300 μm can be obtained. The thickness of plastinated sections can be further reduced by grinding. Resulting sections of <100 µm are suitable for histological staining and microscopic studies. Anatomical structures of interest in thick plastinate slices can be followed by variable manual grinding in a method referred to as Tissue Tracing Technique (TTT). In addition, the tissue thickness can be adapted to the transparency or darkness of tissue types in different regions of the same plastinated section. The aim of this study was to evaluate the advantages of techniques based on sectioning and grinding of plastinated tissue (E12 ultra-thin and TTT) compared to conventional sheet-forming techniques (E12).
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