Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells.

Tytuł:
Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells.
Autorzy:
Morrell BC; Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Zhang L; Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Schütz LF; Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Perego MC; Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Maylem ERS; Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA.
Spicer LJ; Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, 74078, USA. Electronic address: .
Źródło:
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2019 Dec 01; Vol. 498, pp. 110572. Date of Electronic Publication: 2019 Sep 04.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: Limerick : North Holland Publishing
Original Publication: Amsterdam, North-Holland.
MeSH Terms:
Gene Expression Regulation*
E2F Transcription Factors/*metabolism
Fibroblast Growth Factor 9/*metabolism
Granulosa Cells/*metabolism
Ovarian Follicle/*metabolism
RNA, Messenger/*metabolism
Theca Cells/*metabolism
Animals ; Cattle ; E2F Transcription Factors/genetics ; Female ; Fibroblast Growth Factor 9/genetics ; Granulosa Cells/cytology ; Ovarian Follicle/cytology ; RNA, Messenger/genetics ; Theca Cells/cytology
Contributed Indexing:
Keywords: Cattle; E2F8 transcription factor; Granulosa cell; theca cell
Substance Nomenclature:
0 (E2F Transcription Factors)
0 (Fibroblast Growth Factor 9)
0 (RNA, Messenger)
Entry Date(s):
Date Created: 20190908 Date Completed: 20200624 Latest Revision: 20200624
Update Code:
20240105
DOI:
10.1016/j.mce.2019.110572
PMID:
31493442
Czasopismo naukowe
Overexpression of the transcription factor, E2F8, has been associated with ovarian cancer. Objectives of this study were to determine: 1) if E2F8 gene expression in granulosa cells (GC) and theca cells (TC) change with follicular development, and 2) if E2F8 mRNA abundance in TC and GC is hormonally regulated. Using real-time PCR, E2F8 mRNA abundance in GC and TC was greater (P < 0.05) in small than large follicles. FGF9 induced an increase (P < 0.05) in E2F8 mRNA abundance by 1.6- to 7-fold in large-follicle (8-20 mm) TC and GC as well as in small-follicle (1-5 mm) GC. Abundance of E2F8 mRNA in TC was increased (P < 0.05) with FGF2, FGF9 or VEGFA treatments alone in vitro, and concomitant treatment of VEGFA with FGF9 increased (P < 0.05) abundance of E2F8 mRNA above any of the singular treatments; BMP4, WNT3A and LH were without effect. IGF1 amplified the stimulatory effect of FGF9 on E2F8 mRNA abundance by 2.7-fold. Collectively, our studies show for the first time that follicular E2F8 is developmentally and hormonally regulated indicating that E2F8 may be involved in follicular development.
(Copyright © 2019 Elsevier B.V. All rights reserved.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies