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Tytuł:
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Molecular identification and phytochemical screening of endophytic fungi isolated from Lithospermum officinale L. roots: A new source of shikonin.
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Autorzy:
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Mollaei S; Phytochemical Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. Electronic address: .
Khanehbarndaz O; Phytochemical Laboratory, Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
Gerami-Khashal Z; Department of Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
Ebadi M; Department of Biology, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. Electronic address: .
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Źródło:
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Phytochemistry [Phytochemistry] 2019 Dec; Vol. 168, pp. 112116. Date of Electronic Publication: 2019 Sep 09.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2003- : London : Elsevier
Original Publication: 1961-2003: Oxford : Pergamon Press.
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MeSH Terms:
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Antioxidants/*isolation & purification
Endophytes/*chemistry
Lithospermum/*microbiology
Naphthoquinones/*isolation & purification
Naphthoquinones/*pharmacology
Phytochemicals/*isolation & purification
Antioxidants/chemistry ; Antioxidants/pharmacology ; Biphenyl Compounds/antagonists & inhibitors ; Drug Evaluation, Preclinical ; Naphthoquinones/chemistry ; Phylogeny ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Picrates/antagonists & inhibitors ; Plant Roots/microbiology
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Contributed Indexing:
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Keywords: Boraginaceae; Endophytic fungi; Fusarium tricinctum; Lithospermum officinale; Nectriaceae; Shikonin
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Substance Nomenclature:
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0 (Antioxidants)
0 (Biphenyl Compounds)
0 (Naphthoquinones)
0 (Phytochemicals)
0 (Picrates)
3IK6592UBW (shikonin)
DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
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Entry Date(s):
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Date Created: 20190913 Date Completed: 20191206 Latest Revision: 20191217
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Update Code:
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20240105
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DOI:
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10.1016/j.phytochem.2019.112116
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PMID:
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31513947
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Endophytic fungi are microorganisms located in the inter- or intracellular compartments of plant tissues but with no harmful effects. They are considered a potential source of biological compounds. The present study was conducted to investigate the molecular identification of endophytic fungi isolated from the roots of Lithospermum officinale and their potential production of shikonin. Phylogenetic analysis was performed based on the Internal Transcribed Spacer (ITS) region and the isolates were classified into five genera as follows: Alternaria, Chaetosphaeronema, Fusarium, Mucor, and Trichoderma. The study on the methanol extracts of endophytic fungi indicated that total polyphenol content had a positive relationship with antioxidant activities and the highest antioxidant activity belonged to the methanol extracts of Fusarium tricinctum and Alternaria altenata. Then, to investigate the ability of the fungal isolates to produce shikonin, a naphthoquinone compound with high biological activity, the extracts were subjected to HPLC. The results obtained from HPLC-mass spectrometry showed that shikonin could be produced only by F. tricinctum. Thus, F. tricinctum isolated from the roots of L. officinale can be presented as a new source of shikonin.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
