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Tytuł:
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Arachidonic acid exacerbates diet-induced obesity and reduces bone mineral content without impacting bone strength in growing male rats.
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Autorzy:
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Mak IL; School of Human Nutrition, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, QC, Canada H9X 3V9.
Lavery P; School of Human Nutrition, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, QC, Canada H9X 3V9.
Agellon S; School of Human Nutrition, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, QC, Canada H9X 3V9.
Rauch F; Shriners' Hospital for Children, 1003 Decarie Boulevard, Montreal, QC, Canada H4A 0A9.
Murshed M; Shriners' Hospital for Children, 1003 Decarie Boulevard, Montreal, QC, Canada H4A 0A9; Faculty of Dentistry, McGill University, 3640 rue University, Montreal, QC, Canada H3A 0C7.
Weiler HA; School of Human Nutrition, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, QC, Canada H9X 3V9. Electronic address: .
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Źródło:
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The Journal of nutritional biochemistry [J Nutr Biochem] 2019 Nov; Vol. 73, pp. 108226. Date of Electronic Publication: 2019 Aug 15.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <1996->: New York, NY : Elsevier Science
Original Publication: Stoneham, MA, USA : Butterworths, c1990-
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MeSH Terms:
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Arachidonic Acid/*adverse effects
Bone Density/*drug effects
Bone and Bones/*physiopathology
Diet, High-Fat/*adverse effects
Obesity/*etiology
3T3-L1 Cells ; Adipocytes/drug effects ; Adiposity/drug effects ; Animals ; Arachidonic Acid/administration & dosage ; Biomechanical Phenomena ; Body Composition/drug effects ; Bone Resorption/etiology ; Bone and Bones/drug effects ; Energy Intake ; Fatty Acids/pharmacology ; Femur/drug effects ; Femur/pathology ; Leptin/blood ; Male ; Mice ; Obesity/pathology ; Obesity/physiopathology ; Rats ; Rats, Sprague-Dawley
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Contributed Indexing:
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Keywords: Adipocytes; Arachidonic acid; Bone; Childhood; Diet-induced obesity; Fatty acids; Growth
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Substance Nomenclature:
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0 (Fatty Acids)
0 (Leptin)
27YG812J1I (Arachidonic Acid)
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Entry Date(s):
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Date Created: 20190915 Date Completed: 20201016 Latest Revision: 20201016
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Update Code:
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20240105
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DOI:
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10.1016/j.jnutbio.2019.108226
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PMID:
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31520815
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Long-chain polyunsaturated fatty acids modulate bone mass and adipocyte metabolism. Arachidonic acid (AA, C20:4 n-6) is elevated in obesity and postulated to stimulate bone resorption. This study aimed to determine the effect of AA on bone mass, quality, and adiposity in diet-induced obesity during growth. Male Sprague-Dawley rats (n=42, 4-week) were randomized into groups fed a control diet (CTRL, AIN-93G), high-fat diet (HFD, 35% kcal fat) or HFD + AA (1% w/w diet) for 6 weeks. Body composition, bone mineral density and microarchitecture were measured using dual-energy X-ray absorptiometry and micro-computed tomography. Red blood cell fatty acid profile was measured with gas chromatography. Group differences were evaluated using repeated measures two-way analysis of variance with Tukey-Kramer post hoc testing. Total energy intake did not differ among diet groups. At week 6, HFD + AA had significantly greater body fat % (12%), body weight (6%) and serum leptin concentrations (125%) than CTRL, whereas visceral fat (mass and %, assessed with micro-computed tomography) was increased in both HFD and HFD + AA groups. HFD + AA showed reduced whole body bone mineral content and femur mid-diaphyseal cortical bone cross-sectional area than HFD and CTRL, without impairment in bone strength. Contrarily, HFD + AA had greater femur metaphyseal trabecular vBMD (35%) and bone volume fraction (5%) compared to controls. Inclusion of AA elevated leptin concentrations in male rats. The early manifestations of diet-induced obesity on bone mass were accelerated with AA. Studies of longer duration are needed to clarify the effect of AA on peak bone mass following growth cessation.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
