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Tytuł:
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Distribution of camptothecin biosynthetic intermediates and identification the rate-limiting step of camptothecin biosynthesis.
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Autorzy:
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Sun Y; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, China.
Zhang N; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, China.
Wang C; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, China.
Wei Y; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, China.
Liu J; Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, China.
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Źródło:
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Natural product research [Nat Prod Res] 2021 Jul; Vol. 35 (13), pp. 2170-2177. Date of Electronic Publication: 2019 Sep 19.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Milton Park, UK : Taylor & Francis Health Sciences, c2003-
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MeSH Terms:
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Biosynthetic Pathways*
Camptothecin/*biosynthesis
Camptothecin/chemistry ; Chromatography, High Pressure Liquid ; Kinetics ; Reproducibility of Results
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Contributed Indexing:
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Keywords: Camptothecin; distribution; high performance liquid chromatography; intermediate; rate-limiting step
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Substance Nomenclature:
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XT3Z54Z28A (Camptothecin)
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Entry Date(s):
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Date Created: 20190921 Date Completed: 20210709 Latest Revision: 20210709
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Update Code:
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20240105
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DOI:
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10.1080/14786419.2019.1665252
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PMID:
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31537116
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Two key biosynthetic intermediates (pumiloside and strictosamide) of camptothecin were isolated. A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed to determine four main alkaloid compounds (pumiloside, strictosamide, camptothecin and 10-hydroxycamptothecin) and estimate two minor compounds (deoxypumiloside, 9-methoxycamptothecin) simultaneously in different parts of Camptotheca acuminata , with a good linearity and R 2 > 0.999 for all curves. The results indicated that there was a positive correlation between the two key intermediates (strictosamide and pumiloside) and camptothecin in vivo. The speculation that the root was the synthetic position of camptothecin in vivo was confirmed. The rate-limiting step of camptothecin biosynthesis was estimated the step from pumiloside to deoxypumiloside based on its concentration fall sharply.
