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Tytuł:
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Assessing the mutagens ethylnitrolic acid and 2-methyl-1,4-dinitro-pyrrole in meat products: Sample preparation and simultaneous analysis by LC-MS/MS.
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Autorzy:
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Molognoni L; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Federal de Defesa Agropecuária, São José, SC 88102-600, Brazil; Instituto Catarinense de Sanidade Agropecuária, Florianópolis, SC 88034-000, Brazil; Universidade Federal de Santa Catarina (UFSC), Centro de Ciências Agrárias, Departamento de Ciência e Tecnologia de Alimentos, Rodovia Admar Gonzaga, 1346, Itacorubi, Florianópolis, SC 88034-000, Brazil.
Daguer H; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Federal de Defesa Agropecuária, São José, SC 88102-600, Brazil.
Hoff RB; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Federal de Defesa Agropecuária, São José, SC 88102-600, Brazil.
Rodrigues J; UFSC, Centro de Ciências Físicas e Matemáticas, Departamento de Química, Florianópolis, SC 88040-900, Brazil.
Joussef AC; UFSC, Centro de Ciências Físicas e Matemáticas, Departamento de Química, Florianópolis, SC 88040-900, Brazil.
De Dea Lindner J; Universidade Federal de Santa Catarina (UFSC), Centro de Ciências Agrárias, Departamento de Ciência e Tecnologia de Alimentos, Rodovia Admar Gonzaga, 1346, Itacorubi, Florianópolis, SC 88034-000, Brazil. Electronic address: .
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Źródło:
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Journal of chromatography. A [J Chromatogr A] 2020 Jan 04; Vol. 1609, pp. 460512. Date of Electronic Publication: 2019 Sep 04.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam ; New York : Elsevier, 1993-
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MeSH Terms:
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Analytic Sample Preparation Methods*
Hydroxylamines/*analysis
Meat Products/*analysis
Mutagens/*analysis
Nitriles/*analysis
Pyrroles/*chemistry
Tandem Mass Spectrometry/*methods
Calibration ; Chromatography, Liquid ; Chromatography, Reverse-Phase ; Reproducibility of Results
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Contributed Indexing:
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Keywords: (NZ)-N-(1-nitroethylidene)hydroxylamine; Food control; Preservatives; Random uncertainty; Single-laboratory Validation approach; Systematic uncertainties
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Substance Nomenclature:
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0 (Hydroxylamines)
0 (Mutagens)
0 (Nitriles)
0 (Pyrroles)
0 (ethylnitrolic acid)
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Entry Date(s):
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Date Created: 20190923 Date Completed: 20200227 Latest Revision: 20200227
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Update Code:
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20240105
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DOI:
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10.1016/j.chroma.2019.460512
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PMID:
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31542208
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The simultaneous use of nitrite and sorbate as preservatives in meat products may produce mutagenic compounds such as the ethylnitrolic acid and 2-methyl-1,4-dinitro-pyrrole. We developed a sensitive analytical method with high metrological reliability. After assessing several extraction approaches and chromatographic separation modes, a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) approach was chosen for sample preparation, which were analyzed by reversed-phase liquid chromatography (with C 18 as stationary phase) coupled to tandem mass spectrometry. After validation, we confirmed that this method is fit-for-purpose, since it was applied to the analysis of several meat products. Limits of detection were set from 5 to 20 µg kg -1 . Satisfactory results were obtained for both compounds, such as precision (CV > 20%) and recoveries (77-92%). This method determine these carcinogenic compounds in processed meats, contributing to the preservation of public health and the improvement of food regulation and control.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
