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Tytuł:
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Optimization of silk fibroin membranes for retinal implantation.
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Autorzy:
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Suzuki S; Queensland Eye Institute, South Brisbane, Queensland 4101, Australia.
Shadforth AMA; Queensland Eye Institute, South Brisbane, Queensland 4101, Australia; School of Biomedical Sciences and Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland 4000, Australia.
McLenachan S; Centre for Ophthalmology and Visual Science, The University of Western Australia, Crawley, Western Australia 6009, Australia; Ocular Tissue Engineering Laboratory, Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia 6009, Australia.
Zhang D; Ocular Tissue Engineering Laboratory, Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia 6009, Australia.
Chen SC; Ocular Tissue Engineering Laboratory, Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia 6009, Australia.
Walshe J; Queensland Eye Institute, South Brisbane, Queensland 4101, Australia.
Lidgerwood GE; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, VIC 3002, Australia; Department of Surgery and the Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria 3002, Australia.
Pébay A; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, VIC 3002, Australia; Department of Surgery and the Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria 3002, Australia.
Chirila TV; Queensland Eye Institute, South Brisbane, Queensland 4101, Australia; Science & Engineering Faculty, Queensland University of Technology, 2 George Street, Brisbane, Queensland 4001, Australia; Faculty of Medicine, University of Queensland, Herston, Queensland 4029, Australia; Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Queensland 4072, Australia; Faculty of Science, University of Western Australia, Crawley, Western Australia 6009, Australia.
Chen FK; Centre for Ophthalmology and Visual Science, The University of Western Australia, Crawley, Western Australia 6009, Australia; Ocular Tissue Engineering Laboratory, Lions Eye Institute, 2 Verdun Street, Nedlands, Western Australia 6009, Australia; Department of Ophthalmology, Royal Perth Hospital, Wellington Street, Perth, Western Australia 6000, Australia.
Harkin DG; Queensland Eye Institute, South Brisbane, Queensland 4101, Australia; School of Biomedical Sciences and Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland 4000, Australia. Electronic address: .
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Źródło:
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Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 Dec; Vol. 105, pp. 110131. Date of Electronic Publication: 2019 Aug 29.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier
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MeSH Terms:
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Membranes, Artificial*
Visual Prosthesis*
Fibroins/*chemistry
Animals ; Bombyx ; Human Embryonic Stem Cells/cytology ; Humans ; Intercellular Signaling Peptides and Proteins/metabolism ; Permeability ; Phagocytosis ; Rats ; Retinal Pigment Epithelium/cytology ; Solutions ; Spectroscopy, Fourier Transform Infrared ; Tensile Strength
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Contributed Indexing:
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Keywords: Biocompatibility; Horseradish peroxidase; Phagocytosis; Pigment epithelium-derived factor; Poly(ethylene glycol); Retinal implant; Retinal pigment epithelium; Silk fibroin; Vascular endothelial growth factor
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Substance Nomenclature:
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0 (Intercellular Signaling Peptides and Proteins)
0 (Membranes, Artificial)
0 (Solutions)
9007-76-5 (Fibroins)
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Entry Date(s):
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Date Created: 20190925 Date Completed: 20200206 Latest Revision: 20200206
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Update Code:
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20240105
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DOI:
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10.1016/j.msec.2019.110131
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PMID:
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31546376
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Silk fibroin membrane displays potential for ocular tissue reconstruction as demonstrated by its ability to support a functioning retinal pigment epithelium (RPE) in vitro. Nevertheless, translation of these findings to the clinic will require the use of membranes that can be readily handled and implanted into diseased retinas, with minimal impact on the surrounding healthy tissue. To this end, we optimized the physical properties of fibroin membranes to enable surgical handling during implantation into the retina, without compromising biocompatibility or permeability. Our central hypothesis is that optimal strength and permeability can be achieved by combining the porogenic properties of poly(ethylene glycol) (PEG) with the crosslinking properties of horseradish peroxidase (HRP). Our study reveals that PEG used in conjunction with HRP enables the production of fibroin membranes with superior handling properties to conventional fibroin membranes. More specifically, the modified membranes could be more easily implanted into the retinas of rats and displayed good evidence of biocompatibility. Moreover, the modified membranes retained the ability to support construction of functional RPE derived from pluripotent stem cells. These findings pave the way for preclinical studies of RPE-implantation using the optimized fibroin membranes.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
