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Tytuł:
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The mobile FOX AmpC beta-lactamases originated in Aeromonas allosaccharophila.
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Autorzy:
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Ebmeyer S; Center for Antibiotic Resistance Research, SE-40530 Göteborg, Sweden; Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, SE-41346 Göteborg, Sweden.
Kristiansson E; Center for Antibiotic Resistance Research, SE-40530 Göteborg, Sweden; Mathematical Sciences, Chalmers University of Technology and the University of Gothenburg, SE-41296 Göteborg, Sweden.
Larsson DGJ; Center for Antibiotic Resistance Research, SE-40530 Göteborg, Sweden; Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, SE-41346 Göteborg, Sweden. Electronic address: .
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Źródło:
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International journal of antimicrobial agents [Int J Antimicrob Agents] 2019 Dec; Vol. 54 (6), pp. 798-802. Date of Electronic Publication: 2019 Oct 07.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam : Elsevier Science Publishers, c1991-
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MeSH Terms:
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Aeromonas/*enzymology
Aeromonas/*genetics
Anti-Bacterial Agents/*pharmacology
Bacterial Proteins/*genetics
Drug Resistance, Bacterial/*genetics
beta-Lactamases/*genetics
Bacterial Proteins/classification ; DNA Transposable Elements ; Enterobacteriaceae/genetics ; Gene Expression Regulation, Bacterial ; Genome, Bacterial ; Phylogeny ; beta-Lactamases/classification
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Contributed Indexing:
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Keywords: Aeromonas; AmpC; Antibiotic resistance; FOX
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Substance Nomenclature:
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0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
0 (DNA Transposable Elements)
EC 3.5.2.6 (AmpC beta-lactamases)
EC 3.5.2.6 (beta-Lactamases)
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SCR Organism:
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Aeromonas allosaccharophila
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Entry Date(s):
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Date Created: 20191011 Date Completed: 20200416 Latest Revision: 20200416
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Update Code:
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20240104
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DOI:
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10.1016/j.ijantimicag.2019.09.017
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PMID:
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31600552
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Objective: It is important to understand the origins of antibiotic resistance genes so that risks associated with the emergence of novel resistance genes can be assessed and managed. The chromosomal ampC gene (CAV-1) of Aeromonas caviae (A. caviae) has been reported as the origin of mobile FOX cephalosporinases. The recent identification of A. caviae as the origin of MOX-2 cephalosporinases and the comparably great sequence divergence between FOX and MOX genes makes it unlikely that both genes arose from the same species. Therefore, this study investigated the origin of FOX cephalosporinases using large-scale genomics.
Methods: Publicly available genomes and plasmids were searched for FOX-like genes. Synteny and nucleotide identities of the identified FOX-like genes and their genetic environments were compared and a phylogenetic tree was generated.
Results: FOX-like genes were identified in > 230 Aeromonas genomes and in 46 Enterobacteriaceae isolates. Analysis of the genomic context of CAV-1 revealed a truncated insertion sequence directly upstream of the ampC gene. The chromosomal ampCs of A. caviae (n = 31) were 75-78% identical to CAV-1. In contrast, CAV-1, mobile FOX genes and their context were 95-98% similar to the chromosomal ampC-locus of Aeromonas allosaccharophila (A. allosaccharophila) (n = 6). The A. allosaccharophila ampCs formed a monophyletic branch with mobile FOX genes, whereas the A. caviae ampCs clustered with mobile MOX genes.
Conclusions: These findings show that FOX cephalosporinases originate not in A. caviae, as previously reported, but in A. allosaccharophila, which is a fish pathogen. This finding agrees with the hypothesis that antibiotic use in aquaculture could have contributed to the emergence of FOX genes in human pathogens.
(Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)
