Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Application of spherical substrate to observe bacterial motility machineries by Quick-Freeze-Replica Electron Microscopy.

Tytuł:
Application of spherical substrate to observe bacterial motility machineries by Quick-Freeze-Replica Electron Microscopy.
Autorzy:
Katayama E; The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, 558-8585, Japan. .; Waseda Research Institute for Science and Engineering, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan. .
Tahara YO; The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, 558-8585, Japan.
Bertin C; The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, 558-8585, Japan.
Shibata S; Molecular Cryo-Electron Microscopy Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Kunigami-gun, Okinawa, 904-0495, Japan.
Źródło:
Scientific reports [Sci Rep] 2019 Oct 14; Vol. 9 (1), pp. 14765. Date of Electronic Publication: 2019 Oct 14.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:
Flavobacterium/*ultrastructure
Mycoplasmataceae/*ultrastructure
Bacterial Physiological Phenomena ; Flavobacterium/cytology ; Flavobacterium/physiology ; Freeze Fracturing/methods ; Microscopy, Electron/methods ; Mycoplasmataceae/cytology ; Mycoplasmataceae/physiology ; Silicon Dioxide/chemistry ; Surface Properties
References:
Eukaryot Cell. 2015 Oct;14(10):1017-42. (PMID: 26253157)
EMBO J. 1996 Sep 16;15(18):4844-51. (PMID: 8890158)
J Struct Biol. 2006 Nov;156(2):342-54. (PMID: 16875842)
J Mol Biol. 1996 Jan 26;255(3):458-75. (PMID: 8568890)
Int J Mol Sci. 2018 May 08;19(5):. (PMID: 29738465)
J Cell Biol. 1980 Jul;86(1):212-34. (PMID: 6893451)
Can J Microbiol. 2002 Jan;48(1):1-6. (PMID: 11888158)
Biophysics (Nagoya-shi). 2014 Nov 12;10:89-97. (PMID: 27493503)
Curr Opin Microbiol. 2016 Feb;29:15-21. (PMID: 26500189)
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2081-5. (PMID: 7892228)
J Cell Biol. 1987 Nov;105(5):2373-82. (PMID: 3680387)
Curr Opin Microbiol. 2015 Dec;28:93-7. (PMID: 26476806)
Biophys J. 2010 Apr 21;98(8):1589-97. (PMID: 20409479)
J Mol Biol. 2016 Jan 29;428(2 Pt A):332-343. (PMID: 26456135)
Curr Opin Microbiol. 2015 Dec;28:72-7. (PMID: 26461123)
Biophys J. 2016 Sep 6;111(5):1008-13. (PMID: 27602728)
Front Microbiol. 2017 Feb 03;8:58. (PMID: 28217108)
J Cell Biol. 2006 Sep 11;174(6):851-62. (PMID: 16954349)
Microbiology (Reading). 2000 Jun;146 ( Pt 6):1311-1320. (PMID: 10846210)
J Bacteriol. 1977 Mar;129(3):1495-501. (PMID: 14925)
J Mol Biol. 1998 May 1;278(2):349-67. (PMID: 9571057)
J Bacteriol. 2011 Feb;193(3):715-22. (PMID: 21097617)
J Bacteriol. 2008 Apr;190(8):2851-7. (PMID: 18281397)
J Neurocytol. 2004 May;33(3):277-85. (PMID: 15475683)
Ultramicroscopy. 2016 Nov;170:24-34. (PMID: 27522477)
J Cell Sci. 1984 Jul;69:167-78. (PMID: 6386835)
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11913-8. (PMID: 8876237)
Science. 2002 Nov 8;298(5596):1209-13. (PMID: 12424373)
J Mol Biol. 1983 Sep 5;169(1):155-95. (PMID: 6684695)
J Cell Biol. 1979 May;81(2):275-300. (PMID: 38256)
J Electron Microsc (Tokyo). 2011;60 Suppl 1:S3-29. (PMID: 21844598)
J Bacteriol. 2004 Jul;186(13):4382-6. (PMID: 15205441)
Ultramicroscopy. 2008 Feb;108(3):239-55. (PMID: 18054167)
Substance Nomenclature:
7631-86-9 (Silicon Dioxide)
Entry Date(s):
Date Created: 20191016 Date Completed: 20201030 Latest Revision: 20210110
Update Code:
20240105
PubMed Central ID:
PMC6791848
DOI:
10.1038/s41598-019-51283-w
PMID:
31611568
Czasopismo naukowe
3-D Structural information is essential to elucidate the molecular mechanisms of various biological machineries. Quick-Freeze Deep-Etch-Replica Electron Microscopy is a unique technique to give very high-contrast surface profiles of extra- and intra-cellular apparatuses that bear numerous cellular functions. Though the global architecture of those machineries is primarily required to understand their functional features, it is difficult or even impossible to depict side- or highly-oblique views of the same targets by usual goniometry, inasmuch as the objects (e.g. motile microorganisms) are placed on conventional flat substrates. We introduced silica-beads as an alternative substrate to solve such crucial issue. Elongated Flavobacterium and globular Mycoplasmas cells glided regularly along the bead's surface, similarly to those on a flat substrate. Quick-freeze replicas of those cells attached to the beads showed various views; side-, oblique- and frontal-views, enabling us to study not only global but potentially more detailed morphology of complicated architecture. Adhesion of the targets to the convex surface could give surplus merits to visualizing intriguing molecular assemblies within the cells, which is relevant to a variety of motility machinery of microorganisms.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies