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Tytuł:
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Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material.
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Autorzy:
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Dou WT; Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. .
Liu LF; Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. and National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd, Shanghai 201203, P. R. China. .
Gao J; Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. and National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd, Shanghai 201203, P. R. China. .
Zang Y; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd, Shanghai 201203, P. R. China. .
Chen GR; Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. .
Field RA; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
James TD; Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.
Li J; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd, Shanghai 201203, P. R. China. .
He XP; Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. .
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Źródło:
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Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2019 Oct 31; Vol. 55 (88), pp. 13235-13238.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Cambridge : Royal Society of Chemistry
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MeSH Terms:
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Optical Imaging*
Biomarkers, Tumor/*analysis
Endothelial Protein C Receptor/*analysis
Fluorescent Dyes/*chemistry
Peptides/*chemistry
Triple Negative Breast Neoplasms/*diagnostic imaging
Cell Line, Tumor ; Female ; Humans ; MCF-7 Cells ; Molecular Structure ; Particle Size ; Spectrometry, Fluorescence ; Surface Properties
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Substance Nomenclature:
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0 (Biomarkers, Tumor)
0 (Endothelial Protein C Receptor)
0 (Fluorescent Dyes)
0 (Peptides)
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Entry Date(s):
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Date Created: 20191018 Date Completed: 20191121 Latest Revision: 20200108
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Update Code:
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20240105
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DOI:
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10.1039/c9cc06399d
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PMID:
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31621698
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Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.
Erratum in: Chem Commun (Camb). 2020 Jan 14;56(4):664. (PMID: 31848536)