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Tytuł pozycji:

Ultrasensitive fluorescence detection of sequence-specific DNA via labeling hairpin DNA probes for fluorescein o-acrylate polymers.

Tytuł:
Ultrasensitive fluorescence detection of sequence-specific DNA via labeling hairpin DNA probes for fluorescein o-acrylate polymers.
Autorzy:
Yang X; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China.
Liu Q; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, PR China.
Wen D; Pharmacy College, Henan University of Chinese Medicine, Zhengzhou, 450008, PR China.
Gao M; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China; Laboratories of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China.
Zhang D; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China; Laboratories of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China.
Jin Q; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China; Laboratories of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China.
Kong J; School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, PR China. Electronic address: .
Zhang J; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China; Laboratories of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, Jiangsu Province, PR China. Electronic address: .
Źródło:
Analytica chimica acta [Anal Chim Acta] 2019 Dec 11; Vol. 1088, pp. 144-149. Date of Electronic Publication: 2019 Aug 27.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Amsterdam : Elsevier
Original Publication: Amsterdam.
MeSH Terms:
Inverted Repeat Sequences*
Limit of Detection*
Acrylates/*chemistry
Biosensing Techniques/*methods
DNA/*analysis
DNA Probes/*chemistry
Fluorescein/*chemistry
Base Sequence ; DNA/blood ; DNA/chemistry ; DNA/genetics ; DNA Probes/genetics ; Humans ; Polymers/chemistry ; Spectrometry, Fluorescence
Contributed Indexing:
Keywords: Activators generated by electron transfer for atom transfer radical polymerization (AGET ATRP); Click chemistry; DNA detection; Fluorescence microscopy
Substance Nomenclature:
0 (Acrylates)
0 (DNA Probes)
0 (Polymers)
9007-49-2 (DNA)
TPY09G7XIR (Fluorescein)
Entry Date(s):
Date Created: 20191019 Date Completed: 20200217 Latest Revision: 20200217
Update Code:
20240105
DOI:
10.1016/j.aca.2019.08.060
PMID:
31623710
Czasopismo naukowe
Sensitive detection of DNA is conducive to enhance the accuracy of diseases diagnosis and risk prediction. In this work, we report the use of activators generated by electron transfer for atom transfer radical polymerization (AGET ATRP) as a novel on-chip amplification strategy for the fluorescence detection of DNA. More specifically, the target DNA was captured by the on-chip immobilized hairpin DNA probes. Upon hybridization, exposed 3'-N 3 of the hairpin was used to attach AGET ATRP initiators onto the silicon surface by click chemistry. Then, numerous fluorescent labeling linked to the end of the probes via the formation of long chain polymers of fluorescein o-acrylate, which in turn amplified the fluorescence signal for DNA detection. Under optimal conditions, it showed a good linear range from 100 fM to 1 μM in DNA detection, with the limit of detection as low as 4.3 fM. Moreover, this strategy showed good detection performance in complex real serum samples, the fluorescence intensity of 0.1 nM tDNA in 1% fetal bovine serum samples was 97.6% of that in Tris-EDTA buffer. Based on its high sensitivity, reduced cost and simplicity, the proposed signal amplification strategy displays translational potential in clinical application.
(Copyright © 2019 Elsevier B.V. All rights reserved.)

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