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Tytuł:
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Acetylcholine biosensor based on the electrochemical functionalization of graphene field-effect transistors.
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Autorzy:
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Fenoy GE; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CONICET. Diagonal 113 and 64, La Plata, 1900, Argentina; Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, 25 de Mayo y Francia, 1 Piso, 1650, Buenos Aires, Argentina.
Marmisollé WA; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CONICET. Diagonal 113 and 64, La Plata, 1900, Argentina.
Azzaroni O; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CONICET. Diagonal 113 and 64, La Plata, 1900, Argentina; CEST-UNLP Partner Lab for Bioelectronics, Diagonal 64 y 113, La Plata, 1900, Argentina. Electronic address: .
Knoll W; CEST - Competence Center for Electrochemical Surface Technologies, Konrad Lorenz Strasse 24, 3430, Tulln, Austria; Austrian Institute of Technology, Donau-City-Strasse 1, 1220, Vienna, Austria.
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Źródło:
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Biosensors & bioelectronics [Biosens Bioelectron] 2020 Jan 15; Vol. 148, pp. 111796. Date of Electronic Publication: 2019 Oct 22.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Oxford : Elsevier Advanced Technology
Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
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MeSH Terms:
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Transistors, Electronic*
Acetylcholine/*analysis
Biosensing Techniques/*instrumentation
Graphite/*chemistry
Polymers/*chemistry
Acetylcholinesterase/chemistry ; Aniline Compounds/chemistry ; Benzylamines/chemistry ; Enzymes, Immobilized/chemistry ; Equipment Design ; Oxidation-Reduction ; Polymerization ; Water/analysis
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Contributed Indexing:
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Keywords: Acetylcholine; Acetylcholinesterase; Electropolymerization; Field-effect transistor; Graphene; gFETs
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Substance Nomenclature:
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0 (Aniline Compounds)
0 (Benzylamines)
0 (Enzymes, Immobilized)
0 (Polymers)
0 (graphene oxide)
059QF0KO0R (Water)
7782-42-5 (Graphite)
EC 3.1.1.7 (Acetylcholinesterase)
N9YNS0M02X (Acetylcholine)
SIR7XX2F1K (aniline)
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Entry Date(s):
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Date Created: 20191031 Date Completed: 20200618 Latest Revision: 20200618
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Update Code:
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20240105
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DOI:
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10.1016/j.bios.2019.111796
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PMID:
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31665672
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We present a new strategy of Acetylcholinesterease (AchE) immobilization on graphene field-effect transistors (gFETs) for building up Acetylcholine sensors. This method is based on the electrosynthesis of an amino moiety-bearing polymer layer on the graphene channel. The film of the copolymer poly(3-amino-benzylamine-co-aniline) (PABA) does not only provide the suitable electrostatic charge and non-denaturing environment for enzyme immobilization, but it also improves the pH sensitivity of the gFETs (from 40.8 to 56.3 μA/pH unit), probably due to its wider effective pKa distribution. The local pH changes caused by the enzyme-catalyzed hydrolysis produce a shift in the Dirac point of the gFETs to more negative values, which are evidenced as differences in the gFET conductivity and thereby constituted the signal transduction mechanism of the modified transistors. In this way, the constructed biosensors showed a LOD of 2.3 μM and were able to monitor Ach in the range from 5 to 1000 μM in a flow configuration. Moreover, they showed a sensitivity of -26.6 ± 0.7 μA/Ach decade and also exhibited a very low RSD of 2.6%, revealing good device-to-device reproducibility. The biosensors revealed an excellent selectivity to interferences known to be present in the extracellular milieu, and the response to Ach was recovered by 97.5% after the whole set of interferences injected. Finally, the biosensors showed a fast response time, with an average value of 130 s and a good long-term response.
(Copyright © 2019 Elsevier B.V. All rights reserved.)