-
Tytuł:
-
Optimization of an artificial cellulase cocktail for high-solids enzymatic hydrolysis of cellulosic materials with different pretreatment methods.
-
Autorzy:
-
Du J; College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang 277160, China; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Liang J; College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang 277160, China.
Gao X; College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang 277160, China.
Liu G; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China. Electronic address: .
Qu Y; State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
-
Źródło:
-
Bioresource technology [Bioresour Technol] 2020 Jan; Vol. 295, pp. 122272. Date of Electronic Publication: 2019 Oct 17.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
-
MeSH Terms:
-
Cellulase*
Cellulose*
Adsorption ; Cellulose 1,4-beta-Cellobiosidase ; Hydrolysis ; Lignin ; beta-Glucosidase
-
Contributed Indexing:
-
Keywords: Cellulase; Cellulose conversion; High-solids enzymatic hydrolysis; Lignocellulosic biomass; Pretreatment methods
-
Substance Nomenclature:
-
9004-34-6 (Cellulose)
9005-53-2 (Lignin)
EC 3.2.1.21 (beta-Glucosidase)
EC 3.2.1.4 (Cellulase)
EC 3.2.1.91 (Cellulose 1,4-beta-Cellobiosidase)
-
Entry Date(s):
-
Date Created: 20191101 Date Completed: 20191119 Latest Revision: 20191119
-
Update Code:
-
20240104
-
DOI:
-
10.1016/j.biortech.2019.122272
-
PMID:
-
31669875
-
Optimization of the composition of cellulase mixtures is an effective strategy to improve their hydrolytic efficiency and reduce protein demand during enzymatic degradation of lignocelluloses. In this study, the mixture design method was used to optimize the ratios of endoglucanase II (EG II), cellobiohydrolase I (CBH I) and β-glucosidase I (BG I) from Penicillium oxalicum in an artificial cellulase mixture for the hydrolysis of six different cellulosic materials. The optimal composition of enzyme mixture was distinctly different among not only cellulosic materials with different pretreatment methods but hydrolyses at different solids concentrations. CBH I was most critical for the hydrolysis of two acid-pretreated materials, probably due to its strong adsorption on lignin. A higher proportion of EG II was needed for the hydrolysis of ammonium sulfite pretreated wheat straw. The requirements of specific cellulase components were more pronounced at high solids concentrations, highlighting the importance of considering solids loading when optimizing cellulase cocktails.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
