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Tytuł:
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Highly ordered 3D electrochemical DNA biosensor based on dual orientation controlled rolling motor and graftable tetrahedron DNA.
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Autorzy:
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Liu Z; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Lei S; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Zou L; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Li G; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Xu L; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China.
Ye B; College of Chemistry, Zhengzhou University, Zhengzhou, 450001, PR China. Electronic address: .
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Źródło:
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Biosensors & bioelectronics [Biosens Bioelectron] 2020 Jan 01; Vol. 147, pp. 111759. Date of Electronic Publication: 2019 Oct 03.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Oxford : Elsevier Advanced Technology
Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
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MeSH Terms:
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Biosensing Techniques*
Electrochemical Techniques*
DNA/*isolation & purification
Oligonucleotides/*chemistry
DNA/chemistry ; DNA/genetics ; Limit of Detection ; Nucleic Acid Amplification Techniques ; Oligonucleotides/genetics
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Contributed Indexing:
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Keywords: 3D electrochemical DNA biosensor; Dual orientation control; Graftable tetrahedron DNA; Highly ordered; Rolling motor
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Substance Nomenclature:
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0 (Oligonucleotides)
9007-49-2 (DNA)
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Entry Date(s):
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Date Created: 20191101 Date Completed: 20200401 Latest Revision: 20200401
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Update Code:
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20240104
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DOI:
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10.1016/j.bios.2019.111759
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PMID:
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31670180
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Herein, a robust and highly ordered three-dimensional electrochemical DNA (3D E-DNA) biosensor was proposed, and its orientation was controlled from top down by poly adenine oligonucleotides (polyA-ODNs)-mediated rolling motor (PRM) and graftable tetrahedron DNA (GTD). The GTD with a grafting domain was immobilized on the electrode surface to construct a well-organized sensing interface and controlled the orientation and distribution of the whole system at the "bottom" of this biosensor. The polyA-ODNs regulated the direction and density of the leg DNA attached on PRM at the "top" of the biosensor. The motion was achieved through the target induced cyclic cleaving, which triggered the motor rolling rather than walk. Impressively, the duplex strand DNA (dsDNA) formed after grafting, as a girder, provided a stable support to the soft long single strand (ssDNA), which facilitated the formation of the catalytic center, elevated the efficiency of the rolling cleavage. Under the optimal conditions, the designed biosensor exhibited a lower limit of 0.17 nM and wide linear range from 0.5 nM to 1.5 μM for adenosine rapid detection. Unique dual orientation regulated characteristics of the system increased the probability hybridization enormously and improved the motion efficiency significantly, which offered new avenue of DNA nanomachines development in biosensor platform.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
