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Tytuł:
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α-Irradiation setup for primary human cell cultures.
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Autorzy:
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Maier A; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.
Wiedemann J; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.
Adrian JA; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.; Physics Department, Technical University, Darmstadt, Germany.
Dornhecker M; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.; Biophysics Department, Goethe University, Frankfurt, Germany.
Zipf A; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.; Physics Department, Technical University, Darmstadt, Germany.
Kraft-Weyrather W; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.
Kraft G; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.
Richter S; Fraunhofer-Institut für Mikrostruktur von Werkstoffen und Systemen IMWS, Halle, Germany.
Teuscher N; Fraunhofer-Institut für Mikrostruktur von Werkstoffen und Systemen IMWS, Halle, Germany.
Fournier C; GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany.
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Źródło:
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International journal of radiation biology [Int J Radiat Biol] 2020 Feb; Vol. 96 (2), pp. 206-213. Date of Electronic Publication: 2019 Nov 18.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: London ; New York : Taylor & Francis, [c1988-
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MeSH Terms:
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Alpha Particles*
Americium*
Linear Energy Transfer*
Primary Cell Culture*
Animals ; CHO Cells ; Cell Line ; Collagen/chemistry ; Cricetinae ; Cricetulus ; Dose-Response Relationship, Radiation ; Histones/metabolism ; Humans ; Keratinocytes/cytology ; Oxygen/metabolism ; Polyethylene Terephthalates ; Radiometry ; Reproducibility of Results
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Contributed Indexing:
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Keywords: Keratinocytes; high LET; irradiation; radon; α-particles
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Substance Nomenclature:
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0 (H2AX protein, human)
0 (Histones)
0 (Polyethylene Terephthalates)
9007-34-5 (Collagen)
S88TT14065 (Oxygen)
VW92PHU2UY (Americium)
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Entry Date(s):
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Date Created: 20191105 Date Completed: 20200423 Latest Revision: 20200423
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Update Code:
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20240104
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DOI:
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10.1080/09553002.2020.1683641
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PMID:
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31682776
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Purpose: We present an α-irradiation setup for the irradiation of primary human cell cultures under controlled conditions using 241 Am α-particles. Materials and Methods: To irradiate samples with α-particles in a valid manner, a reliable dosimetry is a great challenge because of the short α-range and the complex energy spectrum. Therefore, the distance between α-source and sample must be minimal. In the present setup, this is achieved by cells growing on a 2 μm thick biaxially-oriented polyethylene terephthalate (boPET) foil which is only 2.7 mm apart from the source. A precise and reproducible exposure time is realized through a mechanical shutter. The fluence, energy spectra and the corresponding linear energy transfer are determined by the source geometry and the material traversed. They were measured and calculated, yielding a dose rate of 8.2 ± 2.4 Gy/min. To improve cell growth on boPET foils, they were treated with air plasma. This treatment increased the polarity and thus the ability of cells attaching to the surface of the foil. Several tests including cell growth, staining for a marker of DNA double-strand breaks and a colony-forming assay were performed and confirm our dosimetry. Conclusion: With our setup, it is possible to irradiate cell cultures under defined conditions with α-particles. The plasma-treated foil is suitable for primary human cell cultures as shown in cell experiments, confirming also the expected number of particle traversals.
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