Characterisation of Crandell-Rees Feline Kidney (CRFK) cells as mesenchymal in phenotype.

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Tytuł:: Characterisation of Crandell-Rees Feline Kidney (CRFK) cells as mesenchymal in phenotype.
Autorzy:: Lawson JS; Comparative Biomedical Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK. Electronic address: .
Syme HM; Clinical Sciences and Services, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts, AL9 7TA, UK.
Wheeler-Jones CPD; Comparative Biomedical Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK.
Elliott J; Comparative Biomedical Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK.
Źródło:: Research in veterinary science [Res Vet Sci] 2019 Dec; Vol. 127, pp. 99-102. Date of Electronic Publication: 2019 Oct 30.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: London : British Veterinary Association
Original Publication: Oxford.
MeSH Terms:: Cats*
Epithelial-Mesenchymal Transition*
Cell Line/*cytology
Stromal Cells/*cytology
Animals ; Cell Line/classification ; Epithelial Cells/classification ; Epithelial Cells/cytology ; Kidney ; Phenotype ; Stromal Cells/classification
References:: J Int Med Res. 2012;40(2):475-85. (PMID: 22613408)
Res Vet Sci. 2015 Dec;103:60-9. (PMID: 26679797)
J Virol. 2011 Jun;85(12):5840-52. (PMID: 21490095)
Di Yi Jun Yi Da Xue Xue Bao. 2005 Jun;25(6):712-4, 717. (PMID: 15958317)
Am J Physiol. 1997 Oct;273(4):F563-74. (PMID: 9362334)
Vet Pathol. 2017 May;54(3):490-510. (PMID: 28129098)
In Vitro. 1973 Nov-Dec;9(3):176-85. (PMID: 4130570)
New Microbiol. 2011 Jan;34(1):25-32. (PMID: 21344143)
J Feline Med Surg. 2019 Aug;21(8):780-787. (PMID: 30345862)
Res Vet Sci. 2010 Apr;88(2):294-9. (PMID: 19822338)
J Histochem Cytochem. 1969 Aug;17(8):517-26. (PMID: 5816239)
J Vet Intern Med. 2016 Mar-Apr;30(2):602-10. (PMID: 26948860)
J Virol. 2007 Jul;81(13):7048-60. (PMID: 17459941)
In Vitro Cell Dev Biol Anim. 2004 Jul-Aug;40(7):211-5. (PMID: 15638703)
Am J Vet Res. 2005 Mar;66(3):506-11. (PMID: 15822597)
BMC Vet Res. 2018 Mar 9;14(1):76. (PMID: 29523136)
PLoS One. 2018 Aug 23;13(8):e0202577. (PMID: 30138414)
J Vet Intern Med. 2010 Mar-Apr;24(2):306-13. (PMID: 20136712)
Contributed Indexing:: Keywords: Cat; Cell line; Renal; Vaccination
Entry Date(s):: Date Created: 20191105 Date Completed: 20200221 Latest Revision: 20200221
Update Code:: 20240104
PubMed Central ID:: PMC6863388
DOI:: 10.1016/j.rvsc.2019.10.012
PMID:: 31683198
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The Crandell-Rees Feline Kidney Cell (CRFK) is an immortalised cell line derived from the feline kidney that is utilised for the growth of certain vaccinal viruses. Confusion exists as to whether CRFK are epithelial or mesenchymal in phenotype. The aim of this study was to characterise CRFK cells via immunofluorescence, enzyme cytochemistry, western blotting, RT-qPCR for S100A4 and comparison to primary feline proximal tubular epithelial cells (FPTEC) and feline cortical fibroblasts (FCF). CRFK cells were of fusiform morphology and appeared similar to FCF. CRFK expressed the mesenchymal intermediate filament (IF) protein vimentin together with two cell adhesion molecules associated with feline fibroblasts (CD29 and CD44), and lacked expression of the epithelial IF cytokeratin, myogenic IF desmin and endothelial marker von Willebrand factor (vWF). In addition, CRFK did not demonstrate brush border enzyme activity typical of FPTEC. S100A4 gene expression, implicated in both neoplastic transformation and epithelial to mesenchymal transition, was highly upregulated in CRFK in comparison to the primary feline renal cells. CRFK appear phenotypically similar to fibroblasts, rather than tubular epithelial cells, and may have undergone neoplastic transformation or epithelial-to-mesenchymal transition after extensive passaging. This finding may have potential implications for future research utilising this cell line.
(Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)

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