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Tytuł pozycji:

Assessing the fate of recombinant plant DNA in rabbit's tissues fed genetically modified cotton.

Tytuł :
Assessing the fate of recombinant plant DNA in rabbit's tissues fed genetically modified cotton.
Autorzy :
Shahid AA; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Salisu IB; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.; Department of Animal Science, Faculty of Agriculture, Federal University Dutse, Dutse, Nigeria.
Yaqoob A; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Rao AQ; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Ullah I; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Husnain T; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
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Źródło :
Journal of animal physiology and animal nutrition [J Anim Physiol Anim Nutr (Berl)] 2020 Jan; Vol. 104 (1), pp. 343-351. Date of Electronic Publication: 2019 Nov 07.
Typ publikacji :
Clinical Trial, Veterinary; Journal Article
Język :
English
Imprint Name(s) :
Original Publication: [Berlin] : Blackwell Science, c2001-
MeSH Terms :
Plants, Genetically Modified*
Cottonseed Oil/*administration & dosage
DNA, Plant/*metabolism
DNA, Recombinant/*metabolism
Gossypium/*genetics
Rabbits/*metabolism
Animal Feed/analysis ; Animal Nutritional Physiological Phenomena ; Animals ; Cottonseed Oil/metabolism ; Diet/veterinary
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Contributed Indexing :
Keywords: PCR; fate of tDNA; feeding; rabbits; tissues; transgenic cottonseed meal
Substance Nomenclature :
0 (Cottonseed Oil)
0 (DNA, Plant)
0 (DNA, Recombinant)
Entry Date(s) :
Date Created: 20191109 Date Completed: 20201116 Latest Revision: 20201116
Update Code :
20210210
DOI :
10.1111/jpn.13243
PMID :
31701592
Czasopismo naukowe
Various feeding studies have been conducted with the different species of animals to evaluate the possible transfer of transgenic DNA (tDNA) from genetically modified (GM) feed into the animal tissues. However, the conclusions drawn from most of such studies are sometimes controversial. Thus, in the present study, an attempt has been made to evaluate the fate of tDNA in rabbits raised on GM cotton-based diet through PCR analysis of the DNA extracted specifically from blood, liver, kidney, heart and intestine (jejunum). A total of 48 rabbits were fed a mixed diet consisting variable proportions of transgenic cottonseeds meal (i.e. 0% w/w, 20% w/w, 30% w/w and 40% w/w) for 180 days. The presence of transgenic DNA fragments (Cry1Ac, Cry2A and CP4 EPSPS) or plant endogenous gene (Sad1) was traced in those specific tissues and organs. The presence of β-actin (ACTB) was also monitored as an internal control. Neither the transgenic fragments (459 bp of Cry1Ac gene, 167 bp of Cry2A gene and111 bp of CP4 EPSPS gene) nor cotton endogenous reference gene (155 bp of Sad1) could be detected in any of the DNA samples extracted from the rabbit's tissues in both control and transgenic groups. However, 155 bp fragment of the rabbit's reference gene (ACTB) was recovered in all the DNA samples extracted from rabbit tissues. The results obtained from this study revealed that both plant endogenous and transgenic DNA fragments have same fate in rabbit's tissues and were efficiently degraded in the gastrointestinal tract (GIT).
(© 2019 Blackwell Verlag GmbH.)

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