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Tytuł:
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Enhancement of host infectivity, immunity, and protective efficacy by addition of sodium bicarbonate antacid to oral vaccine formulation of live attenuated Salmonella secreting Brucella antigens.
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Autorzy:
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Hewawaduge C; College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, Iksan, Republic of Korea.
Senevirathne A; College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, Iksan, Republic of Korea.
Lee JH; College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, Iksan, Republic of Korea. Electronic address: .
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Źródło:
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Microbial pathogenesis [Microb Pathog] 2020 Jan; Vol. 138, pp. 103857. Date of Electronic Publication: 2019 Nov 06.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: London ; Orlando : Academic Press, c1986-
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MeSH Terms:
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Brucella Vaccine*/administration & dosage
Brucella Vaccine*/biosynthesis
Brucella Vaccine*/chemistry
Sodium Bicarbonate*/administration & dosage
Vaccines, Attenuated*/administration & dosage
Vaccines, Attenuated*/biosynthesis
Vaccines, Attenuated*/chemistry
Administration, Oral ; Animals ; Antigens, Bacterial/immunology ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/biosynthesis ; Bacterial Vaccines/chemistry ; Immunity, Cellular ; Immunity, Humoral ; Intestines/immunology ; Intestines/microbiology ; Mice ; Microorganisms, Genetically-Modified ; Salmonella typhimurium/genetics ; Salmonella typhimurium/immunology ; Transformation, Bacterial ; Vaccination/methods
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Contributed Indexing:
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Keywords: Antacid agent; Brucella; Humoral and cell-mediated immunity; Oral vaccination; Salmonella typhimurium
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Substance Nomenclature:
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0 (Antigens, Bacterial)
0 (Bacterial Vaccines)
0 (Brucella Vaccine)
0 (Vaccines, Attenuated)
8MDF5V39QO (Sodium Bicarbonate)
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Entry Date(s):
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Date Created: 20191110 Date Completed: 20200605 Latest Revision: 20200605
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Update Code:
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20240105
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DOI:
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10.1016/j.micpath.2019.103857
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PMID:
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31705999
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In the present study, the importance of sodium bicarbonate antacid as an agent for an orally delivered attenuated Salmonella strain secreting Brucella antigens Cu-Zn superoxide dismutase (SodC) and outer membrane protein 19 (Omp19) as a live vaccine candidate against Brucella infection was investigated. First, Brucella antigens SodC and Omp19 were cloned into a prokaryotic constitutive expression vector, pJHL65. Then secretion of proteins was verified after transformation into an attenuated Salmonella typhimurium (ST) strain, JOL1800 (Δlon, ΔcpxR, Δasd, ΔrfaL), using western blot analysis. Mice were orally inoculated with phosphate-buffered saline (PBS) or with a co-mixture Salmonella secreting each antigens at a 1:1 ratio, each containing 1 × 10 8 CFU/mouse with and without sodium bicarbonate treatment. For antacid treatment, 1.3% w/v sodium bicarbonate was orally administered 30 min before and immediately after immunization with the Salmonella formulation. Humoral and cell-mediated immune responses were evaluated to investigate the efficacy of sodium bicarbonate in an oral formulation. The results indicated that addition of sodium bicarbonate to the vaccine significantly increased (P < 0.05) levels of anti-Brucella-specific systemic IgG responses, lymphocyte proliferation, and CD4 + T cell responses, indicating induction of a mixed Th1-Th2 response. Immunohistochemical assays and bacterial enumeration in intestinal samples also indicated that administration of sodium bicarbonate enhanced colonization of Salmonella. These results indicate that ingestion of the Salmonella formulation with sodium bicarbonate can enhance colonization of Salmonella and induce a significant protective immune response against Brucella compared with a formulation without sodium bicarbonate. Thus, incorporation of sodium bicarbonate as an antacid buffer is highly recommended for this oral live vaccine.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
