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Tytuł:
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A rhodol-hemicyanine based ratiometric fluorescent probe for real-time monitoring of glutathione dynamics in living cells.
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Autorzy:
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Ren M; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Wang L; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Lv X; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Sun Y; Institute of Chemical Biology and Clinical Application at the First Affiliated Hospital, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, P. R. China.
Chen H; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Zhang K; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Wu Q; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Bai Y; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
Guo W; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. .
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Źródło:
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The Analyst [Analyst] 2019 Dec 21; Vol. 144 (24), pp. 7457-7462. Date of Electronic Publication: 2019 Nov 11.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Cambridge : Royal Society of Chemistry
Original Publication: London : Chemical Society
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MeSH Terms:
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Optical Imaging*
Carbocyanines/*chemistry
Fluorescent Dyes/*chemistry
Glutathione/*metabolism
Xanthones/*chemistry
A549 Cells ; Humans ; Spectrometry, Fluorescence
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Substance Nomenclature:
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0 (Carbocyanines)
0 (Fluorescent Dyes)
0 (Xanthones)
0 (rhodol)
0 (tetramethylene hemicyanine)
GAN16C9B8O (Glutathione)
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Entry Date(s):
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Date Created: 20191112 Date Completed: 20191230 Latest Revision: 20191230
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Update Code:
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20240105
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DOI:
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10.1039/c9an01852b
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PMID:
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31710053
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Glutathione (GSH) plays crucial roles in various physiological and pathological processes. The fluctuation of the GSH level is closely associated with a variety of diseases and cellular functions. Hence, it is important to real-time monitor the fluctuation of GSH in living cells. In this work, we presented a rhodol-hybridized hemicyanine fluorophore (RdH) as a selective, rapid-response, ratiometric, and reversible fluorescent probe for intracellular GSH (t 1/2 = 89 ms, K d = 1.42 mM). The imaging assays in living cells revealed that RdH could be used to real-time monitor GSH dynamics in A549 cells under a laser scanning confocal microscope by ratiometric fluorescence changes.