Inhibition of microRNA-150-5p alleviates cardiac inflammation and fibrosis via targeting Smad7 in high glucose-treated cardiac fibroblasts.

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Tytuł:: Inhibition of microRNA-150-5p alleviates cardiac inflammation and fibrosis via targeting Smad7 in high glucose-treated cardiac fibroblasts.
Autorzy:: Che H; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.; Translational Medicine Research and Cooperation Center of Northern China, Heilongjiang Academy of Medical Sciences, Harbin, Heilongjiang, China.
Wang Y; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Li Y; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Lv J; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Li H; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Liu Y; Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China.
Dong R; Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China.
Sun Y; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Xu X; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Zhao J; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Wang L; Department of Endocrinology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Źródło:: Journal of cellular physiology [J Cell Physiol] 2020 Nov; Vol. 235 (11), pp. 7769-7779. Date of Electronic Publication: 2019 Nov 11.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: New York, NY : Wiley-Liss
Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology.
MeSH Terms:: Diabetic Cardiomyopathies/*metabolism
Fibroblasts/*pathology
Glucose/*toxicity
MicroRNAs/*metabolism
Smad7 Protein/*metabolism
Animals ; Cells, Cultured ; Diabetic Cardiomyopathies/pathology ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibrosis ; Gene Expression Regulation ; Hyperglycemia/metabolism ; Hyperglycemia/pathology ; Inflammation/chemically induced ; Inflammation/metabolism ; Inflammation/pathology ; Rats ; Rats, Sprague-Dawley ; Signal Transduction/drug effects ; Signal Transduction/physiology
References:: Camelliti, P., Borg, T. K., & Kohl, P. (2005). Structural and functional characterisation of cardiac fibroblasts. Cardiovascular Research, 65(1), 40-51. https://doi.org/10.1016/j.cardiores.2004.08.020.
Chen, Z., Stelekati, E., Kurachi, M., Yu, S., Cai, Z., Manne, S., … Wherry, E. J. (2017). miR-150 regulates memory CD8 T cell differentiation via c-Myb. Cell Reports, 20(11), 2584-2597. https://doi.org/10.1016/j.celrep.2017.08.060.
DiDonato, J. A., Mercurio, F., & Karin, M. (2012). NF-kappaB and the link between inflammation and cancer. Immunological Reviews, 246(1), 379-400. https://doi.org/10.1111/j.1600-065X.2012.01099.x.
Dillmann, W. H. (2019). Diabetic cardiomyopathy. Circulation Research, 124(8), 1160-1162. https://doi.org/10.1161/CIRCRESAHA.118.314665.
Geng, H., & Guan, J. (2017). MiR-18a-5p inhibits endothelial-mesenchymal transition and cardiac fibrosis through the Notch2 pathway. Biochemical and Biophysical Research Communications, 491(2), 329-336. https://doi.org/10.1016/j.bbrc.2017.07.101.
Guo, Y., Gupte, M., Umbarkar, P., Singh, A. P., Sui, J. Y., Force, T., … Lal, H. (2017). Entanglement of GSK-3beta, beta-catenin and TGF-beta1 signaling network to regulate myocardial fibrosis. Journal of Molecular and Cellular Cardiology, 110, 109-120. https://doi.org/10.1016/j.yjmcc.2017.07.011.
Hayashi, H., Abdollah, S., Qiu, Y., Cai, J., Xu, Y. Y., Grinnell, B. W., … Falb, D. (1997). The MAD-related protein Smad7 associates with the TGFbeta receptor and functions as an antagonist of TGFbeta signaling. Cell, 89(7), 1165-1173.
Huang, Y., Tan, D., Xiao, J., Li, Q., Zhang, X., & Luo, Z. (2018). miR-150 contributes to the radioresistance in nasopharyngeal carcinoma cells by targeting glycogen synthase kinase-3beta. Journal of Cancer Research and Therapeutics, 14(1), 111-118. https://doi.org/10.4103/jcrt.JCRT_682_17.
Ju, B., Nie, Y., Yang, X., Wang, X., Li, F., Wang, M., … Zhang, H. (2019). miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells. Journal of Cellular and Molecular Medicine, 23(6), 3824-3832. https://doi.org/10.1111/jcmm.14210.
Kong, P., Christia, P., & Frangogiannis, N. G. (2014). The pathogenesis of cardiac fibrosis. Cellular and Molecular Life Science, 71(4), 549-574. https://doi.org/10.1007/s00018-013-1349-6.
Lee, W. S., & Kim, J. (2017). Diabetic cardiomyopathy: Where we are and where we are going. Korean Journal of Internal Medicine, 32(3), 404-421. https://doi.org/10.3904/kjim.2016.208.
Li, Y., Ren, W., Wang, X., Yu, X., Cui, L., Li, X., … Shi, B. (2019). MicroRNA-150 relieves vascular remodeling and fibrosis in hypoxia-induced pulmonary hypertension. Biomedicine and Pharmacotherapy, 109, 1740-1749. https://doi.org/10.1016/j.biopha.2018.11.058.
Lin, N., Ji, Z., & Huang, C. (2017). Smad7 alleviates glomerular mesangial cell proliferation via the ROS-NF-kappaB pathway. Experimental Cell Research, 361(2), 210-216. https://doi.org/10.1016/j.yexcr.2017.10.003.
MacKenna, D., Summerour, S. R., & Villarreal, F. J. (2000). Role of mechanical factors in modulating cardiac fibroblast function and extracellular matrix synthesis. Cardiovascular Research, 46(2), 257-263.
Meng, S., Yang, F., Wang, Y., Qin, Y., Xian, H., Che, H., … Wang, L. (2019). Silymarin ameliorates diabetic cardiomyopathy via inhibiting TGF-beta1/Smad signaling. Cell Biology International, 43(1), 65-72. https://doi.org/10.1002/cbin.11079.
Murtaza, G., Virk, H. U. H., Khalid, M., Lavie, C. J., Ventura, H., Mukherjee, D., … Paul, T. K. (2019). Diabetic cardiomyopathy - A comprehensive updated review. Progress in Cardiovascular Diseases, 62(4), 315-326. https://doi.org/10.1016/j.pcad.2019.03.003.
Qiu, Z., He, Y., Ming, H., Lei, S., Leng, Y., & Xia, Z. Y. (2019). Lipopolysaccharide (LPS) aggravates high glucose- and hypoxia/reoxygenation-induced injury through activating ROS-dependent NLRP3 inflammasome-mediated pyroptosis in H9C2 cardiomyocytes. Journal of Diabetes Research, 2019, 8151836. https://doi.org/10.1155/2019/8151836.
Sang, W., Wang, Y., Zhang, C., Zhang, D., Sun, C., Niu, M., … Xu, K. (2016). MiR-150 impairs inflammatory cytokine production by targeting ARRB-2 after blocking CD28/B7 costimulatory pathway. Immunology Letters, 172, 1-10. https://doi.org/10.1016/j.imlet.2015.11.001.
Tan, Z., Jia, J., & Jiang, Y. (2018). MiR-150-3p targets SP1 and suppresses the growth of glioma cells. Bioscience Reports, 38(3), BSR20180019. https://doi.org/10.1042/BSR20180019.
Travers, J. G., Kamal, F. A., Robbins, J., Yutzey, K. E., & Blaxall, B. C. (2016). Cardiac fibrosis: The fibroblast awakens. Circulation Research, 118(6), 1021-1040. https://doi.org/10.1161/CIRCRESAHA.115.306565.
Wu, H. Y., Wu, J. L., & Ni, Z. L. (2019). Overexpression of microRNA-202-3p protects against myocardial ischemia-reperfusion injury through activation of TGF-beta1/Smads signaling pathway by targeting TRPM6. Cell Cycle, 18(5), 621-637. https://doi.org/10.1080/15384101.2019.1580494.
Xing, S. S., Tan, H. W., Bi, X. P., Zhong, M., Zhang, Y., & Zhang, W. (2008). Felodipine reduces cardiac expression of IL-18 and perivascular fibrosis in fructose-fed rats. Molecular Medicine, 14(7-8), 395-402. https://doi.org/10.2119/2008-00024.Xing.
Yamamoto, Y., & Gaynor, R. B. (2001). Therapeutic potential of inhibition of the NF-kappaB pathway in the treatment of inflammation and cancer. Journal of Clinical Investigation, 107(2), 135-142. https://doi.org/10.1172/JCI11914.
Yan, X., Liu, Z., & Chen, Y. (2009). Regulation of TGF-beta signaling by Smad7. Acta Biochim Biophys Sin (Shanghai), 41(4), 263-272.
Yu, Q., Vazquez, R., Khojeini, E. V., Patel, C., Venkataramani, R., & Larson, D. F. (2009). IL-18 induction of osteopontin mediates cardiac fibrosis and diastolic dysfunction in mice. American Journal of Physiology: Heart and Circulatory Physiology, 297(1), H76-85. https://doi.org/10.1152/ajpheart.01285.2008.
Yuan, Y., Das, S. K., & Li, M. (2018). Vitamin D ameliorates impaired wound healing in streptozotocin-induced diabetic mice by suppressing NF-kappaB-mediated inflammatory genes. Bioscience Reports, 38(2), https://doi.org/10.1042/BSR20171294.
Zhou, X., Jiang, L., Fan, G., Yang, H., Wu, L., Huang, Y., … Li, J. (2019). Role of the ciRS-7/miR-7 axis in the regulation of proliferation, apoptosis and inflammation of chondrocytes induced by IL-1beta. International Immunopharmacology, 71, 233-240. https://doi.org/10.1016/j.intimp.2019.03.037.
Contributed Indexing:: Keywords: TGF-β1/Smad; diabetic cardiomyopath; fibrosis; inflammation; microRNA-150-5p
Substance Nomenclature:: 0 (MIRN150 microRNA, rat)
0 (MicroRNAs)
0 (Smad7 Protein)
0 (Smad7 protein, rat)
IY9XDZ35W2 (Glucose)
Entry Date(s):: Date Created: 20191112 Date Completed: 20210308 Latest Revision: 20210308
Update Code:: 20240105
DOI:: 10.1002/jcp.29386
PMID:: 31710102
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Hyperglycemia-induced cardiac fibrosis is a prominent characteristic of diabetic cardiomyopathy. Changes in proinflammatory cytokines have been shown to lead to cardiac fibrosis in patients with diabetes mellitus. This study aimed to investigate the role of miR-150-5p in mediating cardiac inflammation and fibrosis in cardiac fibroblasts (CFs). Herein, we found that high-glucose (HG) treatment significantly induced cardiac inflammation, as manifested by increased proinflammatory cytokine production (IL-1β) and NF-κB activity in CFs. Moreover, HG markedly aggravated cardiac fibrosis and increased levels of fibrotic markers (CTGF, FN, α-SMA) and extracellular matrix proteins (Col-I, Col-III) in CFs. At the same time, HG disturbed the TGF-β1/Smad signaling pathway, as evidenced by increases in TGF-β1 and p-Smad2/3 levels and decreases in Smad7 levels in CFs. Furthermore, we found that miR-150-5p was upregulated by HG, which negatively regulated Smad7 expression at the posttranscription level. Further study demonstrated that cardiac inflammation and fibrosis in CFs were corrected following miR-150-5p knockdown, but exacerbated by miR-150-5p overexpression. These data indicated that miR-150-5p inhibition could ameliorate NF-κB-related inflammation and TGF-β1/Smad-induced cardiac fibrosis through targeting Smad7. Thus, miR-150-5p may be a novel promising target for treating diabetic cardiomyopathy.
(© 2019 Wiley Periodicals, Inc.)

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