Metabolomics of soybean green stem and foliar retention (GSFR) disease using mass spectrometry and molecular networking.

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Tytuł:: Metabolomics of soybean green stem and foliar retention (GSFR) disease using mass spectrometry and molecular networking.
Autorzy:: Zanzarin DM; Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.; Graduate Program in Cell Biology, State University of Maringá, Maringá, PR, Brazil.
Hernandes CP; Graduate Program in Cell Biology, State University of Maringá, Maringá, PR, Brazil.
Leme LM
Silva E; Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.
Porto C; Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.; Master in Science, Technology and Food Safety, Cesumar Institute of Science, Technology, and Innovation-ICETI, University Center of Maringá-UNICESUMAR, Maringá, PR, Brazil.
Martin do Prado R; Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.; Department of Animal Science, State University of Maringá, Maringá, PR, Brazil.
Meyer MC; Brazilian Agricultural Research Corporation-Embrapa Soja, Londrina, PR, Brazil.
Favoreto L; Minas Gerais State Agricultural Research Corporation-Epamig Oeste, Uberaba, MG, Brazil.
Nunes EO; Brazilian Agricultural Research Corporation-Embrapa Soja, Londrina, PR, Brazil.
Pilau EJ; Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.; Graduate Program in Cell Biology, State University of Maringá, Maringá, PR, Brazil.
Źródło:: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2020 Sep; Vol. 34 Suppl 3, pp. e8655. Date of Electronic Publication: 2020 Feb 11.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Chichester : John Wiley And Sons Ltd
Original Publication: London, UK : Heyden, c1987-
MeSH Terms:: Metabolomics/*methods
Plant Roots/*parasitology
Glycine max/*metabolism
Glycine max/*parasitology
Tandem Mass Spectrometry/*methods
Animals ; Chromatography, High Pressure Liquid ; Host-Parasite Interactions ; Plant Diseases/parasitology ; Plant Roots/metabolism ; Secondary Metabolism ; Tylenchida/pathogenicity
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Grant Information:: 0673/13-CT-Infra-FINEP Araucaria Foundation; 155675/2016-8 Araucaria Foundation; 168384/2017-5-CNPq Araucaria Foundation; 401590/2014-3-CNPq Araucaria Foundation
Entry Date(s):: Date Created: 20191114 Date Completed: 20210705 Latest Revision: 20231213
Update Code:: 20240105
DOI:: 10.1002/rcm.8655
PMID:: 31721333
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Rationale: The nematode Aphelenchoides besseyi is the causal agent of green stem and foliar retention, a soybean disease recently described in Brazil. This condition can reduce soybean yield by up to 100%. However, little is known about chemical interactions between the plant and pathogen. Therefore, this work aimed to investigate metabolites from healthy soybean roots and from soybean roots that were inoculated with A. besseyi.
Methods: A. besseyi were multiplied in vitro with Fusarium sp. colonies in Petri dishes for 25 days, and were axenically inoculated into hydroponics healthy soybean plants. The metabolites were extracted from the roots of healthy and A. besseyi-infected plants 16 days post-inoculation. These extracts were analyzed using an untargeted metabolomic method with an ultra-high-performance liquid chromatography/electrospray ionization /tandem mass spectrometry (UHPLC/ESI-MS/MS) and molecular networking approach.
Results: Roots from infected plants showed morphological alterations such as shrinkage, darkening, and arching. Similarly, they also showed an increased presence of flavonoids, compared with healthy roots. Compounds such as neobavaisoflavone, glycitin, genistin, and genistein were putatively identified and had greater intensity in inoculated roots. These compounds are linked to the defensive mechanisms in plants against nematodes. Moreover, coumaric acid, also exclusively putatively identified in inoculated roots, shows activity related to inhibition of root growth.
Conclusions: Liquid chromatography, mass spectrometry, and molecular networking approaches proved to be a powerful tool for the metabolomic study of GSFR. This study showed metabolomics differences of protective substances in the roots, evidencing a quick response of the plant to the attack of A. besseyi.
(© 2019 John Wiley & Sons, Ltd.)

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