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Tytuł:
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CARM1 is heterogeneous in mouse four-cell embryo and important to blastocyst development.
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Autorzy:
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Sun H; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Su J; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Wu T; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Wang F; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Kang J; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Zhang J; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Xing X; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Cheng Y; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Zhang Y; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
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Źródło:
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Reproduction (Cambridge, England) [Reproduction] 2020 Jan; Vol. 159 (1), pp. 91-104.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica
Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001-
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MeSH Terms:
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Embryonic Development*
Gene Expression Regulation, Developmental*
Blastocyst/*metabolism
Blastomeres/*metabolism
Embryo, Mammalian/*metabolism
Protein-Arginine N-Methyltransferases/*metabolism
Animals ; Blastocyst/cytology ; Blastomeres/cytology ; Cell Differentiation ; DNA Methylation ; Embryo, Mammalian/cytology ; Female ; Histones/genetics ; Histones/metabolism ; Mice ; Protein-Arginine N-Methyltransferases/genetics
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Substance Nomenclature:
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0 (Histones)
EC 2.1.1.319 (Protein-Arginine N-Methyltransferases)
EC 2.1.1.319 (coactivator-associated arginine methyltransferase 1)
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Entry Date(s):
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Date Created: 20191114 Date Completed: 20201030 Latest Revision: 20201030
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Update Code:
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20240105
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DOI:
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10.1530/REP-19-0405
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PMID:
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31721723
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Coactivator-associated arginine methyltransferase 1 (CARM1) is a type I arginine methyltransferase that methylates the arginine residues of histone and nonhistone. Carm1 regulates various cellular processes, including transcriptional regulation, mRNA processing, cellular proliferation, and differentiation. Blastomeres with high Carm1 expression levels show cleavage tendency to inner cell mass (ICM) in mouse embryos. However, details about the factors for CARM1 distribution in mouse early embryos and the role of Carm1 in blastocyst development remain unclear. Here, the endonuclear distribution of CARM1 protein was heterogeneous between blastomeres from the late four-cell stage to the blastocyst stage. The heterogeneity of CARM1 distribution in blastomeres at the late four-cell stage was randomly obtained from two-cell stage embryos. From the four-cell stage to morula, CARM1 in individual blastomere remained heterogeneous. In the blastocyst stage, CARM1 protein level in ICM was much higher than that in trophoblast. We found that microRNA (miRNA) miR-181a is an important regulator for Carm1 distribution at the late four-cell stage. The ratio of heterogeneous embryos was reduced in all the embryos when miR-181a was inhibited. CARM1 inhibition reduced the level of symmetrical histone H3 arginine-26 dimethylation and impaired blastocyst development. Silencing Carm1 reduced cell number and increased cell apoptosis at the blastocyst stage. These results show a CARM1 heterogeneous distribution from the four-cell embryos to the blastocysts. miR-181a regulates the control of CARM1 heterogeneous distribution in the four-cell-stage embryos, and CARM1 is an important protein in regulating blastocyst development.