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Tytuł:
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Cathepsin B Turning Bioluminescence "On" for Tumor Imaging.
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Autorzy:
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Ni Y; Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry , University of Science and Technology of China , 96 Jinzhai Road , Hefei , Anhui 230026 , China.
Hai Z; Institutes of Physical Science and Information Technology , Anhui University , 110 Jiulong Road , Hefei , Anhui 230601 , China.
Zhang T; School of Life Sciences , University of Science and Technology of China , 443 Huangshan Road , Hefei , Anhui 230027 , China.
Wang Y; Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry , University of Science and Technology of China , 96 Jinzhai Road , Hefei , Anhui 230026 , China.
Yang Y; Center of Advanced Analysis & Gene Sequencing , Zhengzhou University , 100 Kexue Road , Zhengzhou , Henan 450001 , China.
Zhang S; Center of Advanced Analysis & Gene Sequencing , Zhengzhou University , 100 Kexue Road , Zhengzhou , Henan 450001 , China.
Liang G; Hefei National Laboratory of Physical Sciences at Microscale, Department of Chemistry , University of Science and Technology of China , 96 Jinzhai Road , Hefei , Anhui 230026 , China.
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Źródło:
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Analytical chemistry [Anal Chem] 2019 Dec 03; Vol. 91 (23), pp. 14834-14837. Date of Electronic Publication: 2019 Nov 19.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, American Chemical Society.
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MeSH Terms:
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Biomarkers, Tumor/*genetics
Breast Neoplasms/*diagnostic imaging
Cathepsin B/*genetics
Diagnostic Imaging/*methods
Molecular Probes/*pharmacokinetics
Animals ; Biomarkers, Tumor/antagonists & inhibitors ; Biomarkers, Tumor/metabolism ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cathepsin B/antagonists & inhibitors ; Cathepsin B/metabolism ; Cell Line, Tumor ; Dipeptides/pharmacology ; Female ; Gene Expression ; Heterografts ; Humans ; Luminescent Measurements ; Lysosomes/drug effects ; Lysosomes/enzymology ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Molecular Probes/chemical synthesis
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Substance Nomenclature:
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0 (Biomarkers, Tumor)
0 (CA 074 methyl ester)
0 (Dipeptides)
0 (Molecular Probes)
EC 3.4.22.1 (CTSB protein, human)
EC 3.4.22.1 (Cathepsin B)
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Entry Date(s):
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Date Created: 20191116 Date Completed: 20201013 Latest Revision: 20201013
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Update Code:
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20240105
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DOI:
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10.1021/acs.analchem.9b04254
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PMID:
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31726822
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Cathepsin B (CTSB) is a lysosomal protease, and several human cancers are reported highly expressing CTSB. Many optical methods have been developed for CTSB detection but not a bioluminescence (BL) probe. Herein, a CTSB-specific bioluminescence probe Val-Cit-AL was rationally designed for selectively sensing CTSB activity in vitro with a 67-fold "Turn-On" of BL intensity and an excellent limit of detection. Inhibitory experiments indicated that Val-Cit-AL is capable of sensing CTSB activity in living cells and tumors. We anticipate that Val-Cit-AL might be applied to diagnose CTSB-related diseases in rodent models or evaluate CTSB roles in more biological processes in the near future.
