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Tytuł:
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UVC-Emitting LuPO 4 :Pr Nanoparticles Decrease Radiation Resistance of Hypoxic Cancer Cells.
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Autorzy:
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Müller M; Wellman Center for Photomedicine.
Espinoza S; Department of Chemical Engineering, Münster University of Applied Sciences, Steinfurt, Germany.
Jüstel T; Department of Chemical Engineering, Münster University of Applied Sciences, Steinfurt, Germany.
Held KD; Department of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts.
Anderson RR; Wellman Center for Photomedicine.
Purschke M; Wellman Center for Photomedicine.
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Źródło:
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Radiation research [Radiat Res] 2020 Jan; Vol. 193 (1), pp. 82-87. Date of Electronic Publication: 2019 Nov 18.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Bozeman, MT : Radiation Research Society
Original Publication: Charlottesville, VA : Kluge Carden Jennnings Pub. Co.
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MeSH Terms:
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Lutetium*
Nanoparticles*
Praseodymium*
Ultraviolet Rays*
Radiation Tolerance/*drug effects
Tumor Hypoxia/*radiation effects
A549 Cells ; Cell Proliferation/drug effects ; Cell Proliferation/radiation effects ; Humans ; Radiation Tolerance/radiation effects
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Substance Nomenclature:
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0 (LuPO4:Pr(3+))
5H0DOZ21UJ (Lutetium)
NKN7EZA750 (Praseodymium)
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Entry Date(s):
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Date Created: 20191119 Date Completed: 20200427 Latest Revision: 20200625
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Update Code:
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20240104
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DOI:
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10.1667/RR15491.1
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PMID:
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31738663
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Radiation-resistant hypoxic tumor areas continue to present a major limitation for successful tumor treatment. To overcome this radiation resistance, an oxygen-independent treatment is proposed using UVC-emitting LuPO 4 :Pr 3+ nanoparticles (NPs) and X rays. The uptake of the NPs as well as their effect on cell proliferation was investigated on A549 lung cancer cells by using inverted time-lapse microscopy and transmission electron microscopy. Furthermore, cytotoxicity of the combined treatment of X rays and LuPO 4 :Pr 3+ NPs was assessed under normoxic and hypoxic conditions using the colony formation assay. Transmission electron microscopy (TEM) images showed no NP uptake after 3 h, whereas after 24 h incubation an uptake of NPs was documented. LuPO 4 :Pr 3+ NPs alone caused a concentration-independent cell growth delay within the first 60 h of incubation. The combined treatment with UVC-emitting NPs and X rays reduced the radiation resistance of hypoxic cells by a factor of two to the level of cells under normoxic condition. LuPO 4 :Pr 3+ NPs cause an early growth delay but no cytotoxicity for the tested concentration. The combination of these NPs with X rays increases cytotoxicity of normoxic and hypoxic cancer cells. Hypoxic cells become sensitized to normoxic cell levels.
