Trypsin cleavage sites are highly unlikely to occur in celiac-causing restricted epitopes.

Tytuł:: Trypsin cleavage sites are highly unlikely to occur in celiac-causing restricted epitopes.
Autorzy:: Herman RA; Regulatory and Stewardship, CortevaTM Agriscience, Indianapolis, IN, USA.
Song P; Regulatory and Stewardship, CortevaTM Agriscience, Indianapolis, IN, USA.
Mirsky HP; Regulatory and Stewardship, CortevaTM Agriscience, Johnston, IA, USA.
Źródło:: GM crops & food [GM Crops Food] 2020 Apr 02; Vol. 11 (2), pp. 67-69. Date of Electronic Publication: 2019 Nov 19.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2015- : Philadelphia, PA : Taylor & Francis
Original Publication: Austin, Tex. : Landes Bioscience
MeSH Terms:: Celiac Disease*
Gliadin*
Amino Acid Sequence ; Epitopes ; Humans ; Trypsin
References:: Oral Dis. 2013 Oct;19(7):635-41. (PMID: 23496382)
J Proteome Res. 2008 Jan;7(1):300-5. (PMID: 18067249)
Immunogenetics. 2012 Jun;64(6):455-60. (PMID: 22322673)
Regul Toxicol Pharmacol. 2018 Nov;99:233-237. (PMID: 30266240)
J Proteomics. 2012 Feb 2;75(4):1454-62. (PMID: 22166745)
EFSA J. 2017 Jun 22;15(6):e04862. (PMID: 32728397)
Contributed Indexing:: Keywords: Celiac; bioinformatics; genetically engineered; proteins; trypsin cleavage site
Substance Nomenclature:: 0 (Epitopes)
9007-90-3 (Gliadin)
EC 3.4.21.4 (Trypsin)
Entry Date(s):: Date Created: 20191120 Date Completed: 20200519 Latest Revision: 20210424
Update Code:: 20240104
PubMed Central ID:: PMC7289517
DOI:: 10.1080/21645698.2019.1692612
PMID:: 31743058
: Czasopismo naukowe

To assess risk, the European Food Safety Authority requires that the amino-acid sequences of newly expressed proteins in genetically engineered (GE) crops should be searched for partial matches with 9-mer restricted epitopes known to cause celiac disease. None of the 26 known celiac-causing 9-mer epitopes contain an in-silico predicted trypsin cleavage site. The probability of this occurring by chance alone is 0.000056. Based on the absence of in-silico predicted trypsin cleavage sites within 9-mer epitopes known to cause celiac disease, it can be concluded with very high confidence that true celiac-causing epitopes are highly unlikely to contain in-silico predicted trypsin cleavage sites and that this criterion can reliably be used to exclude the risk that imperfect 9-mer peptide matches within newly expressed proteins from GE crops cause celiac disease.

Erratum in: Plant Signal Behav. 2019 Dec 2;:1. (PMID: 31790626)

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