Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines.

Tytuł:
Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines.
Autorzy:
Fornillos RJC; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.; Natural Sciences Research Institute, University of the Philippines Diliman, P. Velasquez St. Diliman, Quezon City, Philippines.
Sato MO; Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan.
Tabios IKB; College of Medicine, University of the Philippines Manila, Ermita Manilla, Philippines.
Sato M; Graduate School of Health Sciences, Niigata, Japan.
Leonardo LR; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.; Graduate School, University of the East Ramon Magsaysay Memorial Medical Center, Quezon City, Philippines.
Chigusa Y; Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan.
Minamoto T; Graduate School of Human Development and Environment, Kobe University, Tsurukabuto, Nada-ku, Kobe, Japan.
Kikuchi M; Department of Immunogenetics, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan.
Legaspi ER; Medical Zoology Laboratory, Schistosomiasis Research and Training Center, Palo Leyte, Philippines.
Fontanilla IKC; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.; Natural Sciences Research Institute, University of the Philippines Diliman, P. Velasquez St. Diliman, Quezon City, Philippines.
Źródło:
PloS one [PLoS One] 2019 Nov 20; Vol. 14 (11), pp. e0224617. Date of Electronic Publication: 2019 Nov 20 (Print Publication: 2019).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: San Francisco, CA : Public Library of Science
MeSH Terms:
Epidemiological Monitoring*
DNA, Environmental/*isolation & purification
Schistosoma japonicum/*isolation & purification
Schistosomiasis japonica/*prevention & control
Snails/*genetics
Animals ; Cercaria/genetics ; DNA, Environmental/genetics ; Disease Vectors ; Humans ; Philippines/epidemiology ; Schistosoma japonicum/genetics ; Schistosomiasis japonica/epidemiology ; Schistosomiasis japonica/parasitology ; Schistosomiasis japonica/transmission ; Snails/parasitology ; Species Specificity
References:
Mol Ecol. 2012 Apr;21(8):1789-93. (PMID: 22486819)
Environ Sci Technol. 2014;48(3):1819-27. (PMID: 24422450)
Int J Infect Dis. 2018 Nov;76:130-136. (PMID: 30201503)
Lancet. 2014 Jun 28;383(9936):2253-64. (PMID: 24698483)
Int J Infect Dis. 2018 Nov;76:128-129. (PMID: 30266632)
Mol Ecol Resour. 2016 Mar;16(2):415-22. (PMID: 26307935)
Acta Trop. 2002 May;82(2):295-9. (PMID: 12020904)
Biol Lett. 2008 Aug 23;4(4):423-5. (PMID: 18400683)
Trop Biomed. 2019 Jun 1;36(2):402-411. (PMID: 33597401)
Methods Enzymol. 1996;266:383-402. (PMID: 8743695)
Acta Trop. 2017 May;169:1-7. (PMID: 28108370)
J Trop Med. 2012;2012:936128. (PMID: 22518170)
Parasitol Int. 2003 Dec;52(4):409-17. (PMID: 14665400)
Parasitology. 2005 Oct;131(Pt 4):497-500. (PMID: 16174414)
Environ Sci Technol. 2016 Oct 4;50(19):10456-10464. (PMID: 27580258)
Parasit Vectors. 2018 Jun 8;11(1):342. (PMID: 29884202)
Acta Trop. 2015 Jan;141(Pt B):354-60. (PMID: 23583862)
Pathogens. 2019 Sep 23;8(4):. (PMID: 31547610)
Parasitol Int. 2004 Jun;53(2):127-34. (PMID: 15081944)
PLoS Negl Trop Dis. 2008;2(11):e337. (PMID: 19015722)
Mol Ecol. 2017 Nov;26(21):5872-5895. (PMID: 28921802)
Lancet. 2006 Sep 23;368(9541):1106-18. (PMID: 16997665)
Int J Environ Res Public Health. 2017 Aug 30;14(9):. (PMID: 28867814)
Vet Parasitol. 2011 Nov 24;182(1):22-40. (PMID: 21846580)
Methods Mol Biol. 2000;132:115-30. (PMID: 10547834)
Trends Parasitol. 2013 Nov;29(11):548-55. (PMID: 24064438)
Int J Infect Dis. 2014 Jan;18:52-6. (PMID: 24211228)
Emerg Infect Dis. 2013 Dec;19(12):. (PMID: 24295136)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
Trends Parasitol. 2015 Oct;31(10):499-513. (PMID: 26433253)
Am J Hyg. 1947 May;45(3):259-73. (PMID: 20240013)
Appl Environ Microbiol. 2011 Mar;77(6):2192-5. (PMID: 21278276)
Substance Nomenclature:
0 (DNA, Environmental)
Entry Date(s):
Date Created: 20191121 Date Completed: 20200402 Latest Revision: 20231104
Update Code:
20240104
PubMed Central ID:
PMC6867693
DOI:
10.1371/journal.pone.0224617
PMID:
31747401
Czasopismo naukowe
In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection.
Competing Interests: The authors have declared that no competing interests exist.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies