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Tytuł pozycji:

Gynura procumbens ethanol extract and its fractions inhibit macrophage derived foam cell formation.

Tytuł:
Gynura procumbens ethanol extract and its fractions inhibit macrophage derived foam cell formation.
Autorzy:
Manogaran M; Regenerative Medicine Cluster, Malaysia.
Vuanghao L; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Penang, Malaysia.
Mohamed R; Regenerative Medicine Cluster, Malaysia. Electronic address: .
Źródło:
Journal of ethnopharmacology [J Ethnopharmacol] 2020 Mar 01; Vol. 249, pp. 112410. Date of Electronic Publication: 2019 Nov 17.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
MeSH Terms:
Asteraceae/*chemistry
Atherosclerosis/*prevention & control
Foam Cells/*drug effects
Plant Extracts/*pharmacology
ATP Binding Cassette Transporter 1/metabolism ; Animals ; Atherosclerosis/blood ; Ethanol/chemistry ; Foam Cells/metabolism ; Humans ; Interleukin-1beta/metabolism ; Lipoproteins, LDL/metabolism ; Malaysia ; Medicine, Traditional/methods ; Mice ; Plant Extracts/isolation & purification ; Plant Extracts/therapeutic use ; RAW 264.7 Cells ; Scavenger Receptors, Class E/metabolism ; Tumor Necrosis Factor-alpha/metabolism
Contributed Indexing:
Keywords: Atherosclerosis; Foam cell; Gynura procumbens; RAW264.7 cells
Substance Nomenclature:
0 (ABCA1 protein, mouse)
0 (ATP Binding Cassette Transporter 1)
0 (IL1B protein, mouse)
0 (Interleukin-1beta)
0 (Lipoproteins, LDL)
0 (Olr1 protein, mouse)
0 (Plant Extracts)
0 (Scavenger Receptors, Class E)
0 (Tnf protein, mouse)
0 (Tumor Necrosis Factor-alpha)
0 (oxidized low density lipoprotein)
3K9958V90M (Ethanol)
Entry Date(s):
Date Created: 20191121 Date Completed: 20200625 Latest Revision: 20220412
Update Code:
20240104
DOI:
10.1016/j.jep.2019.112410
PMID:
31747560
Czasopismo naukowe
Ethnopharmacology Relevance: Gynura procumbens (Lour.) Merr. displayed cardio-protective effect that may prevent atherogenesis. The primary underlying pathological process of cardiovascular disease is atherosclerosis. Atherosclerotic lesion composed of macrophages, T cells and other immune cells which incorporated with cholesterol that infiltrates from the blood.
Aim of the Study: The present study was performed to determine underlying mechanism of G. procumbens ethanol extract and its fractions such as aqueous, chloroform, ethyl acetate and hexane affect macrophage derived foam cell formation.
Materials and Methods: Lipid droplets accumulation in treated macrophages were visualized by Oil Red O staining while the total cholesterol present in the treated macrophages were measured using Cholestryl Ester quantification assay kit. Enzyme-Linked Immunosorbent Assay (ELISA) were used to detect TNF-α and IL-1β secretion in the supernatant of treated macrophages. Gene expression of Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and ATP-binding cassette transporter A-1 (ABCA-1) in treated macrophages were analyzed using Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR).
Results: G. procumbens ethanol extract and its fractions reduced lipid droplet accumulation and total cholesterol in oxLDL-treated macrophages together with significantly reduction of TNF-α and IL-1β secretions in supernatant oxLDL-treated macrophages. LOX-1 gene expression was significantly reduced when G. procumbens ethanol extract and its fractions were added in oxDL-treated macrophages. In contrast, G. procumbens ethanol extract and its fractions significantly increased the expression of ABCA-1 gene in oxLDL-treated macrophages.
Conclusion: In conclusion, G. procumbens ethanol extract and its fractions inhibit the formation of macrophage derived foam cell by reducing TNF-α and IL-1β expression, which usually highly expressed in atherosclerotic plaques, suppressing scavenger receptor LOX-1 gene that binds oxLDL but induced ABCA-1 gene that mediate lipid efflux from macrophages.
(Copyright © 2019 Elsevier B.V. All rights reserved.)

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