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Tytuł:
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Comparison of Profiling of Hairy Root of Two Tibetan Medicinal Plants Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim.
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Autorzy:
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Lei T; Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
Wang H; Tibetan Medicine Center, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.
Li S; Institute of Soil and Fertilizer, Qinghai University, Xining 810016, Qinghai, China.
Cai X; Institute of Soil and Fertilizer, Qinghai University, Xining 810016, Qinghai, China.
Chen S; Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.; University of Chinese Academy of Sciences, Beijing 100049, China.; Key Laboratory of Crop Molecular Breeding of Qinghai Province, Xining 810008, Qinghai, China.
Cheng T; Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.; University of Chinese Academy of Sciences, Beijing 100049, China.
Shen J; Tibetan Medicine Center, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.
Shi S; Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.
Zhou D; Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.; University of Chinese Academy of Sciences, Beijing 100049, China.; Key Laboratory of Crop Molecular Breeding of Qinghai Province, Xining 810008, Qinghai, China.
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Źródło:
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Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2020; Vol. 21 (6), pp. 516-527.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000-
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MeSH Terms:
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Plant Roots/*chemistry
Plants, Medicinal/*chemistry
Solanaceae/*chemistry
Tropanes/*analysis
Agrobacterium/genetics ; Agrobacterium/growth & development ; Chromatography, High Pressure Liquid ; Genes, Bacterial ; Plant Roots/growth & development ; Plant Roots/microbiology ; Plants, Medicinal/growth & development ; Plants, Medicinal/microbiology ; Solanaceae/growth & development ; Solanaceae/microbiology ; Solanaceous Alkaloids/analysis ; Tibet
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Contributed Indexing:
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Keywords: Agrobacterium rhizogenes; Anisodus tanguticus Maxim.; Przewalskia tangutica Maxim.; growth curve; hairy roots; tropane alkaloids.
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Substance Nomenclature:
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0 (Solanaceous Alkaloids)
0 (Tropanes)
01343Q8EL8 (anisodamine)
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SCR Organism:
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Agrobacterium rhizogenes
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Entry Date(s):
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Date Created: 20191129 Date Completed: 20200713 Latest Revision: 20200713
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Update Code:
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20240104
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DOI:
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10.2174/1389201020666191127125842
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PMID:
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31775597
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Background: Tropane Alkaloids (TAs) are important drugs for curing many diseases in the medical industry.
Methods: To sustainably exploit TA resources in endangered traditional Tibetan herbs, the hairy root (HR) systems of Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim. were compared under the same culture conditions.
Results: The results indicated that both the Agrobacterium rhizogenes strains and explants affected the HR induction frequency, MSU440, A4 and LBA9402 strains could induce hairy roots following infection of cotyledon and hypocotyl of A. tanguticus while LBA9402 could not induce HR on either explants of P. tangutica. The efficiency of LBA9402 was higher than A4 and MSU440 on A. tanguticus and A4 was better strain than MSU440 on P. tangutica. The hypocotyl explant was more suitable for P.tangutica and cotyledon explant was better for A.tangutica with a transformation frequency of 33.3% (P. tangutica) and 82.5% (A. tanguticus), respectively. In a flask reactor system, both the growth curves of HR for two species both appeared to be "S" curve; however, the HR of P. tangutica grew more rapidly than that of A. tanguticus, and the latter accumulated more biomass than the former. As the culture volume increased, the HR proliferation coefficient of both the species increased. HPLC analysis results showed that the content of TAs in the HR of P. tangutica was 257.24mg/100g·DW, which was more than that of A. tanguticus HR (251.08mg/100g·DW), and the anisodamine in the Pt- HR was significantly higher than that in At-HR. Moreover, tropane alkaloids in the HR of the two species were all significantly higher than that of the roots of aseptic seedlings.
Conclusion: Our results suggest that HR of P. tangutica and A. tanguticus both could provide a useful platform for sustainable utilization of two Tibetan medicinal plants in the Qinghai-Tibetan Plateau in the future.
(Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
