In vivo third-harmonic generation microscopy study on vitiligo patients.

Szczegóły
Abstrakt

Tytuł:: In vivo third-harmonic generation microscopy study on vitiligo patients.
Autorzy:: Liao YH; National Taiwan University Hospital, National Taiwan University College of Medicine, Department of D, Taiwan.
Su YH; National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Elect, Taiwan.
Shih YT; National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Elect, Taiwan.
Chen WS; National Taiwan University Hospital, National Taiwan University College of Medicine, Department of P, Taiwan.
Jee SH; National Taiwan University Hospital, National Taiwan University College of Medicine, Department of D, Taiwan.; Cathay General Hospital, Department of Dermatology, Taipei, Taiwan.
Sun CK; National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Elect, Taiwan.
Źródło:: Journal of biomedical optics [J Biomed Opt] 2019 Nov; Vol. 25 (1), pp. 1-13.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Bellingham, WA : Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society, c1996-
MeSH Terms:: Second Harmonic Generation Microscopy/*methods
Vitiligo/*diagnostic imaging
Chromium ; Collagen/metabolism ; Equipment Design ; Humans ; Keratinocytes/metabolism ; Keratinocytes/pathology ; Lasers ; Melanins/metabolism ; Melanocytes/metabolism ; Melanocytes/pathology ; Optical Phenomena ; Second Harmonic Generation Microscopy/instrumentation ; Silicon Compounds ; Skin/diagnostic imaging ; Skin/metabolism ; Skin/pathology ; Ultraviolet Therapy ; Vitiligo/metabolism ; Vitiligo/radiotherapy
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Contributed Indexing:: Keywords: basal keratinocytes; harmonic generation microscopy; melanin; melanocytes; skin; third-harmonic generation; vitiligo
Substance Nomenclature:: 0 (Melanins)
0 (Silicon Compounds)
0 (forsterite)
0R0008Q3JB (Chromium)
9007-34-5 (Collagen)
Entry Date(s):: Date Created: 20191129 Date Completed: 20201013 Latest Revision: 20201013
Update Code:: 20240104
PubMed Central ID:: PMC7008507
DOI:: 10.1117/1.JBO.25.1.014504
PMID:: 31777224
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Melanin is known to provide strong third-harmonic generation (THG) contrast in human skin. With a high concentration in basal cell cytoplasm, THG contrast provided by melanin overshadows other THG sources in human skin studies. For better understanding of the THG signals in keratinocytes without the influence of melanin, an in vivo THG microscopy (THGM) study was first conducted on vitiliginous skin. As a result, the THG-brightness ratio between the melanin-lacking cytoplasm of basal cells and collagen fibers is about 1.106 at the dermal-epidermal junctions of vitiliginous skin, indicating high sensitivity of THGM for the presence of melanin. We further applied the in vivo THGM to assist evaluating the therapeutic outcome from the histopathological point of view for those showed no improvement under narrowband ultraviolet B therapy based on the seven-point Physician Global Assessment score. Our clinical study indicates the high potential of THGM to assist the histopathological assessment of the therapeutic efficacy of vitiligo treatments.

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