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Tytuł:
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Continuous and Discrete Neuron Types of the Adult Murine Striatum.
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Autorzy:
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Stanley G; Program in Biophysics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Gokce O; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians-Universität, Munich, Germany.
Malenka RC; Nancy Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA.
Südhof TC; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: .
Quake SR; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Applied Physics, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: .
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Źródło:
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Neuron [Neuron] 2020 Feb 19; Vol. 105 (4), pp. 688-699.e8. Date of Electronic Publication: 2019 Dec 05.
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Typ publikacji:
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Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: [Cambridge, Mass. : Cell Press, c1988-
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MeSH Terms:
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Corpus Striatum/*cytology
Corpus Striatum/*metabolism
Nerve Net/*cytology
Nerve Net/*metabolism
Neurons/*metabolism
Animals ; Corpus Striatum/chemistry ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Nerve Net/chemistry ; Neurons/chemistry
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Entry Date(s):
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Date Created: 20191210 Date Completed: 20200330 Latest Revision: 20200330
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Update Code:
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20240104
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DOI:
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10.1016/j.neuron.2019.11.004
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PMID:
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31813651
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The mammalian striatum is involved in many complex behaviors and yet is composed largely of a single neuron class: the spiny projection neuron (SPN). It is unclear to what extent the functional specialization of the striatum is due to the molecular specialization of SPN subtypes. We sought to define the molecular and anatomical diversity of adult SPNs using single-cell RNA sequencing (scRNA-seq) and quantitative RNA in situ hybridization (ISH). We computationally distinguished discrete versus continuous heterogeneity in scRNA-seq data and found that SPNs in the striatum can be classified into four major discrete types with no implied spatial relationship between them. Within these discrete types, we find continuous heterogeneity encoding spatial gradients of gene expression and defining anatomical location in a combinatorial mechanism. Our results suggest that neuronal circuitry has a substructure at far higher resolution than is typically interrogated, which is defined by the precise identity and location of a neuron.
Competing Interests: Declaration of Interests The authors declare no competing interests.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
