Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain.

Szczegóły
Abstrakt

Tytuł:: Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain.
Autorzy:: Betts NS; Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.
Dockter C; Carlsberg Research Laboratory, Copenhagen V, Denmark.
Berkowitz O; School of Life Science and ARC Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Melbourne, VIC, Australia.
Collins HM; Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.
Hooi M; Adelaide Glycomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia.
Lu Q; Carlsberg Research Laboratory, Copenhagen V, Denmark.
Burton RA; Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.
Bulone V; Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.; Adelaide Glycomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia.
Skadhauge B; Carlsberg Research Laboratory, Copenhagen V, Denmark.
Whelan J; School of Life Science and ARC Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Melbourne, VIC, Australia.
Fincher GB; Australian Research Council Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.
Źródło:: Journal of experimental botany [J Exp Bot] 2020 Mar 25; Vol. 71 (6), pp. 1870-1884.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Oxford, UK : Oxford University Press,
MeSH Terms:: Gibberellins*
Hordeum*/genetics
Germination ; Seedlings ; Sequence Analysis, RNA
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Contributed Indexing:: Keywords: Hordeum vulgare; Aleurone; RNA-seq; germination; gibberellic acid; scutellum; signal transduction
Substance Nomenclature:: 0 (Gibberellins)
Entry Date(s):: Date Created: 20191211 Date Completed: 20210514 Latest Revision: 20210514
Update Code:: 20240105
PubMed Central ID:: PMC7242073
DOI:: 10.1093/jxb/erz546
PMID:: 31819970
: Czasopismo naukowe

Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain. The model was generated using RNA sequencing transcript data from tissues dissected from intact, germinated grain, which closely match measurements of GA hormones and their metabolites in those tissues. The data show that successful grain germination is underpinned by high concentrations of GA precursors in ungerminated grain, the use of independent metabolic pathways for the synthesis of several bioactive GAs during germination, and a capacity to abort bioactive GA biosynthesis. The most abundant bioactive form is GA1, which is synthesized in the scutellum as a glycosyl conjugate that diffuses to the aleurone, where it stimulates de novo synthesis of a GA3 conjugate and GA4. Synthesis of bioactive GAs in the aleurone provides a mechanism that ensures the hormonal signal is relayed from the scutellum to the distal tip of the grain. The transcript data set of 33 421 genes used to define GA metabolism is available as a resource to analyse other physiological processes in germinated grain.
(© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

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