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Tytuł:
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Effect of refrigeration of blood samples in lithium-heparin tubes on QuantiFERON TB Gold Plus test result.
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Autorzy:
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Uwamino Y; Department of Laboratory Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan; Department of Infectious Diseases, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Sakai A; Department of Laboratory Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Nishimura T; Health Center, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Noguchi M; Department of Laboratory Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Uno S; Department of Infectious Diseases, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Fujiwara H; Department of Infectious Diseases, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Mori M; Health Center, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Wakui M; Department of Laboratory Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Murata M; Department of Laboratory Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Hasegawa N; Department of Infectious Diseases, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan. Electronic address: .
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Źródło:
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Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy [J Infect Chemother] 2020 Mar; Vol. 26 (3), pp. 312-314. Date of Electronic Publication: 2019 Dec 10.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2014- : Amsterdam : Elsevier
Original Publication: Tokyo : New York : Published for the Society by Churchill Livingstone, c1995-
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MeSH Terms:
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Interferon-gamma Release Tests*/methods
Interferon-gamma Release Tests*/standards
Blood Specimen Collection/*methods
Latent Tuberculosis/*diagnosis
Adult ; Aged ; Cold Temperature ; Female ; Heparin ; Humans ; Interferon-gamma/analysis ; Interferon-gamma/metabolism ; Lithium ; Male ; Middle Aged ; Reproducibility of Results
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Contributed Indexing:
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Keywords: Latent tuberculosis infection; QuantiFERON TB Gold Plus; Refrigeration; Sample storage
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Substance Nomenclature:
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82115-62-6 (Interferon-gamma)
9005-49-6 (Heparin)
9FN79X2M3F (Lithium)
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Entry Date(s):
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Date Created: 20191215 Date Completed: 20201207 Latest Revision: 20201214
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Update Code:
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20240105
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DOI:
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10.1016/j.jiac.2019.10.002
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PMID:
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31836288
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The QuantiFERON TB Gold Plus (QFT-Plus) test is a newly approved interferon-gamma releasing assay test for detecting latent tuberculosis. Although blood samples for QFT test can be refrigerated for 48 h in lithium-heparin tubes according to package inserts, no published data are available on the effects of sample refrigeration on the test results. We conducted a clinical study that aimed to elucidate whether sample refrigeration for 48 h affects QFT-Plus test results. We collected 2 blood samples each from 40 participants for QFT-Plus; one sample was refrigerated before incubation for QFT-Plus assay, while the other sample was incubated soon after collection and treated as control. After comparing QFT-Plus test results of refrigerated samples and control samples, the concordance rate and kappa coefficient between them were 95% and 0.90, respectively. Thus, blood samples for QFT-Plus test can be refrigerated for 48 h in lithium-heparin tubes without influencing the test results.
Competing Interests: Declarations of Competing Interest The funder of this study is a QFT-Plus distributor in Japan.
(Copyright © 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
