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Tytuł:
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Genetic diversity and lack of molecular evidence for hemoplasma cross-species transmission between wild and synanthropic mammals from Central-Western Brazil.
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Autorzy:
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Gonçalves LR; Agricultural Microbiology Program at School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
Herrera HM; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
Nantes WAG; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
Santos FM; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
Porfírio GEO; Graduate Program of Natural Resources, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Barreto WTG; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
de Macedo GC; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
Assis WO; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
Campos JBV; Dom Bosco Catholic University, (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
da Silva TMV; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
Mariano LC; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
Barros-Battesti DM; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
Machado RZ; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil.
André MR; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences (FCAV/UNESP), Jaboticabal, São Paulo, Brazil. Electronic address: .
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Źródło:
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Acta tropica [Acta Trop] 2020 Mar; Vol. 203, pp. 105303. Date of Electronic Publication: 2019 Dec 16.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Basel.
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MeSH Terms:
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Animal Diseases/*transmission
Mycoplasma/*genetics
Mycoplasma Infections/*veterinary
Animal Diseases/microbiology ; Animals ; Brazil/epidemiology ; Female ; Genetic Variation ; Mycoplasma/classification ; Mycoplasma Infections/microbiology ; Phylogeny ; Rodentia
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Contributed Indexing:
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Keywords: Capybaras; Hemotropic mycoplasmas; Lice; Opossum; Phylogenetic analysis; Rattus rattus and Ticks
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Entry Date(s):
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Date Created: 20191221 Date Completed: 20200820 Latest Revision: 20200820
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Update Code:
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20240105
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DOI:
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10.1016/j.actatropica.2019.105303
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PMID:
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31857081
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Globally, hemotropic mycoplasmas (hemoplasmas) comprise an emerging or remerging bacteria group that attaches to red blood cells of several mammal's species and in some cases, causing hemolytic anemia. Herein, we assessed the occurrence, genetic diversity, the factors coupled to mammals infection, and the phylogeographic distribution of hemoplasmas in sylvatic and synanthropic mammals and their associated ectoparasites from Brazil. We collected spleen and/or blood samples from synanthropic rodents (Rattus rattus [N = 39] and Mus musculus [N = 9]), sylvatic rodents (Hydrochoerus hydrochaeris [N = 14]) and opossums (Didelphis albiventris [N = 43]). In addition, ticks (Amblyomma spp. [N = 270] and lice (Polyplax spinulosa [N = 6]) specimens were also sampled. Using a PCR targeting the 16S rRNA region, out of 48 small rodents, 14 capybaras and 43 opossums DNA samples, hemoplasma DNA was found in 25%, 50%, and 32.5% animals, respectively. Besides, we reported hemoplasma DNA in Amblyomma sp. (22.2% [2/9]) and lice (100% [2/2]) pools samples from rats, and one female A. sculptum DNA sample (3% [1/33]) obtained from a capybara. Additionally, and in agreement with ML analysis, the network analyses showed a clear phylogenetic separation among the hemoplasmas genotypes found in the different host species sampled, thus, suggesting the absence of cross-species hemoplasmas transmission between the mammals trapped. Finally, using the NTC network analysis, we reported the same 16S rRNA Mycoplasma genotype circulating in Rattus sampled in Brazil, Hungary, and Japan.
Competing Interests: Declaration of competing interest None.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
