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Tytuł:
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MicroRNA-147b promotes lung adenocarcinoma cell aggressiveness through negatively regulating microfibril-associated glycoprotein 4 (MFAP4) and affects prognosis of lung adenocarcinoma patients.
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Autorzy:
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Feng YY; Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, National Clinical Research Center for Cancer, Tianjin 300000, PR China; Department of Pediatric, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, PR China.
Liu CH; Department of Gynaecology and Obstetrics, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, PR China.
Xue Y; Department of Nephrology, Tianjin Medical University General Hospital, Tianjin 300020, PR China.
Chen YY; Department of Gynaecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin 300020, PR China.
Wang YL; Institute of Lung Cancer, Tianjin Medical University General Hospital, Tianjin 300020, PR China.
Wu XZ; Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, National Clinical Research Center for Cancer, Tianjin 300000, PR China; Cancer Center, Tianjin Nankai Hospital, Tianjin 300000, PR China. Electronic address: .
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Źródło:
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Gene [Gene] 2020 Mar 10; Vol. 730, pp. 144316. Date of Electronic Publication: 2019 Dec 26.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
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MeSH Terms:
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Adenocarcinoma of Lung/*genetics
Carrier Proteins/*genetics
Extracellular Matrix Proteins/*genetics
Glycoproteins/*genetics
MicroRNAs/*genetics
Adenocarcinoma/genetics ; Adenocarcinoma of Lung/metabolism ; Adult ; Aged ; Aged, 80 and over ; Carrier Proteins/metabolism ; Case-Control Studies ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation/genetics ; Databases, Genetic ; Disease Progression ; Extracellular Matrix Proteins/metabolism ; Female ; Gene Expression Regulation, Neoplastic/genetics ; Glycoproteins/metabolism ; Humans ; Kaplan-Meier Estimate ; Lung/metabolism ; Lung Neoplasms/genetics ; Male ; MicroRNAs/metabolism ; Middle Aged ; Prognosis
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Contributed Indexing:
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Keywords: Aggressiveness; Lung adenocarcinoma; Microfibril-associated glycoprotein 4; Prognosis; miR-147b
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Substance Nomenclature:
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0 (Carrier Proteins)
0 (Extracellular Matrix Proteins)
0 (Glycoproteins)
0 (MFAP4 protein, human)
0 (MIRN147 microRNA, human)
0 (MicroRNAs)
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Entry Date(s):
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Date Created: 20191230 Date Completed: 20200309 Latest Revision: 20200309
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Update Code:
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20240104
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DOI:
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10.1016/j.gene.2019.144316
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PMID:
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31884109
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Background: Lung adenocarcinoma (LUAD) is widely known as the leading cause of death in patients with lung cancer. Extensive evidence has determined that microRNAs (miRNAs) exert critical effects on various biological processes in tumorigenesis. microRNA-147b (miR-147b) has been reported to serve as an oncogenic molecule in colorectal cancer and hepatocellular carcinoma, however, its prognostic value and biological effect in LUAD remain rare.
Materials and Methods: miR-147b and microfibril-associated glycoprotein 4 (MFAP4) data were collected from The Cancer Genome Atlas (TCGA) database to determine their expression levels in LUAD tissues. Kaplan-Meier method was used to plot the overall survival curves for the prognostic power of miR-147b and MFAP4 identification. Chi-square test was utilized to demonstrate the association between clinical characteristics and miR-147b or MFAP4 in LUAD. Luciferse reporter assay was implemented to identify the correlation between miR-147b and MFAP4. The mRNA and protein levels were detected by qRT-PCR and western blotting, respectively. To explore the effects of miR-147b and its potential mechanism in LUAD, cell counting kit 8 (CCK-8), colony formation and transwell assays were performed in LUAD cells with abnormal expression of miR-147b or/and MFAP4.
Results: Our results showed that miR-147b was up-regulated in LUAD tissues and cell lines, which induced poor outcome. Conversely, MFAP4, the putative target gene of miR-147b, was down-regulated in LUAD. The expression of MFAP4 in LUAD cells was negatively regulated by miR-147b. Results of experiments in vitro revealed that miR-147b could promote cell proliferation, colony formation, invasion and migration, while up-regulation of MFAP4 suppressed the impacts of miR-147b on cell malignant aggressiveness in A549 and Calu-3 cells.
Conclusion: In conclusion, these findings determined that miR-147b contributed to the progression of LUAD via targeting MFAP4. Thus, understanding the potential mechanism of miR-147b/MFAP4 may improve the treatment of cancers, especially LUAD.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
