-
Tytuł:
-
Comprehensive Understanding of the Plasmid-Mediated Colistin Resistance Gene mcr-1 in Aquatic Environments.
-
Autorzy:
-
Zhu L; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
Zhou Z; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
Liu Y; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
Lin Z; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
Shuai X; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
Xu L; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
Chen H; Department of Environmental Engineering, College of Environmental and Resource Sciences , Zhejiang University , Hangzhou 310058 , China.
-
Źródło:
-
Environmental science & technology [Environ Sci Technol] 2020 Feb 04; Vol. 54 (3), pp. 1603-1613. Date of Electronic Publication: 2020 Jan 13.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Washington DC : American Chemical Society
Original Publication: Easton, Pa. : American Chemical Society, c1967-
-
MeSH Terms:
-
Colistin*
Escherichia coli Proteins*
Animals ; Anti-Bacterial Agents ; China ; Drug Resistance, Bacterial ; Ecosystem ; Escherichia coli ; Humans ; Microbial Sensitivity Tests ; Plasmids ; Swine
-
Substance Nomenclature:
-
0 (Anti-Bacterial Agents)
0 (Escherichia coli Proteins)
0 (MCR-1 protein, E coli)
Z67X93HJG1 (Colistin)
-
Entry Date(s):
-
Date Created: 20191231 Date Completed: 20200316 Latest Revision: 20200316
-
Update Code:
-
20240104
-
DOI:
-
10.1021/acs.est.9b05919
-
PMID:
-
31886662
-
The emergence of plasmid-mediated colistin resistance gene mcr-1 has attracted global attention and raised serious concerns about its possible cross-environment dissemination. However, the systematic exploration of mcr-1 both by monitoring and genetic dissection in aquatic environments has not been conducted. This study addresses the gap related to the occurrence and distribution of mcr-1 in watersheds, eastern China. The results showed an abundance of mcr-1 gene in four watersheds, and the highest level of mcr-1 reached 1.8 × 10 9 gene copies per liter of water. Furthermore, the transfer frequencies of the plasmids in isolated Escherichia coli were 2.76 × 10 -6 -6.11 × 10 -4 within genera and minimal inhibitory concentrations of polymyxin resistance were 8-16 mg/L for transconjugants. Mass spectrometry data allowed visualization of the function of mcr-1 expression, rendering bacterial resistance to colistin. The genetic details of six mcr-1 -harboring plasmids in E. coli isolates of aquatic origin were obtained by single-molecule real-time sequencing. These plasmids were closely associated with E. coli strains of pig and human origin, supporting the concept of mcr-1 dissemination across natural environments, livestock farms, and humans. In conclusion, this study provides the first glimpse of the profile of mcr-1 -harboring plasmids and their genetic environment in aquatic ecosystems.