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Tytuł pozycji:

Two-Photon Autofluorescence Imaging of Fixed Tissues: Feasibility and Potential Values for Biomedical Applications.

Tytuł:
Two-Photon Autofluorescence Imaging of Fixed Tissues: Feasibility and Potential Values for Biomedical Applications.
Autorzy:
Li LZ; Department of Radiology & Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. .; Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, USA. .; Institute of Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. .
Masek M; TissueVision, Inc, Somerville, MA, USA.
Wang T; Department of Radiology & Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Xu HN; Department of Radiology & Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Nioka S; Department of Radiology & Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Baur JA; Department of Physiology, Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania, Philadelphia, PA, USA.
Ragan TM; TissueVision, Inc, Somerville, MA, USA. .
Źródło:
Advances in experimental medicine and biology [Adv Exp Med Biol] 2020; Vol. 1232, pp. 375-381.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: 1998- : New York : Kluwer Academic/Plenum Publishers
Original Publication: New York, Plenum Press.
MeSH Terms:
Biomedical Technology*/instrumentation
Biomedical Technology*/methods
NAD*
Optical Imaging*
Animals ; Feasibility Studies ; Flavin-Adenine Dinucleotide ; Heterografts/diagnostic imaging ; Mice ; Oxidation-Reduction ; Photons
References:
Front Neuroanat. 2016 Mar 22;10:31. (PMID: 27047350)
Cancer Res. 2014 Sep 15;74(18):5184-94. (PMID: 25100563)
Cancer Res. 2014 Jun 1;74(11):3067-75. (PMID: 24686167)
J Biol Chem. 1979 Jun 10;254(11):4764-71. (PMID: 220260)
Annu Rev Biomed Eng. 2012;14:351-67. (PMID: 22607264)
Am J Physiol Cell Physiol. 2012 Feb 15;302(4):C629-41. (PMID: 22031602)
J Biomed Opt. 2007 Jan-Feb;12(1):014015. (PMID: 17343490)
J Innov Opt Health Sci. 2014 Mar;7(2):. (PMID: 31827630)
Adv Exp Med Biol. 2009;645:247-53. (PMID: 19227478)
Free Radic Biol Med. 2016 Nov;100:53-65. (PMID: 27519271)
J Innov Opt Health Sci. 2014 Mar 1;7(2):1350045. (PMID: 24917891)
Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6608-13. (PMID: 19366661)
J Biomed Opt. 2016 Nov 1;21(11):114003. (PMID: 27896360)
J Biomed Opt. 2016 Jun 1;21(6):60503. (PMID: 27300321)
J Innov Opt Health Sci. 2009 Oct;2(4):325-341. (PMID: 26015810)
Nat Methods. 2012 Jan 15;9(3):255-8. (PMID: 22245809)
Anal Biochem. 1985 Aug 1;148(2):389-400. (PMID: 4061818)
J Biomed Opt. 2017 Jun 1;22(6):66009. (PMID: 28617923)
Grant Information:
R01 CA191207 United States CA NCI NIH HHS
Contributed Indexing:
Keywords: Autofluorescence; FAD; Fixed tissue; NADH; Two photon imaging
Substance Nomenclature:
0U46U6E8UK (NAD)
146-14-5 (Flavin-Adenine Dinucleotide)
Entry Date(s):
Date Created: 20200102 Date Completed: 20200108 Latest Revision: 20210110
Update Code:
20240104
PubMed Central ID:
PMC7183211
DOI:
10.1007/978-3-030-34461-0_48
PMID:
31893434
Czasopismo naukowe
The value of optical redox imaging (ORI) of cells/tissues based on the intrinsic fluorescences of NADH (nicotinamide adenine dinucleotide) and oxidized flavoproteins (containing flavin adenine dinucleotide, i.e., FAD) has been demonstrated for potential biomedical applications including diagnosis, prognosis, and determining treatment response. However, the Chance redox scanner (a 3D cryogenic tissue imager) is limited by spatial resolution (~50 μm), and tissue ORI using fluorescence microscopy (single or multi-photon) is limited by the light penetration depth. Furthermore, viable or snap-frozen tissues are usually required. In this project, we aimed to study whether ORI may be achieved for unstained fixed tissue using a state-of-the-art modern Serial Two-Photon (STP) Tomography scanner that can rapidly acquire multi-plane images at micron resolution. Tissue specimens of mouse muscle, liver, and tumor xenografts were harvested and fixed in 4% paraformaldehyde (PFA) for 24 h. Tissue blocks were scanned by STP Tomography under room temperature to acquire the autofluorescence signals (NADH channel: excitation 750 nm, blue emission filter; FAD channel: excitation 860 nm, green emission filter). We observed remarkable signals with significant intra-tissue heterogeneity in images of NADH, FAD and redox ratio (FAD/(NADH+FAD)), which are worthy of further investigation for extracting biological information.

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