Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma.

Tytuł:
The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma.
Autorzy:
Feng T; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Zhu Z; Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Jin Y; Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Wang H; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Mao X; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Liu D; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Li Y; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Lu L; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Zuo G; Key Laboratory of Diagnostic Medicine Designated by The Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
Źródło:
Oncology reports [Oncol Rep] 2020 Feb; Vol. 43 (2), pp. 491-502. Date of Electronic Publication: 2019 Dec 31.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: <2003->: Athens : D.A. Spandidos
Original Publication: [Athens, Greece] : National Hellenic Research Foundation, 1994-
MeSH Terms:
Bone Neoplasms/*genetics
Bone Neoplasms/*pathology
MicroRNAs/*genetics
Osteosarcoma/*genetics
Osteosarcoma/*pathology
Zinc Finger E-box-Binding Homeobox 1/*metabolism
Bone Neoplasms/metabolism ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Epithelial-Mesenchymal Transition ; Gene Expression Regulation, Neoplastic ; Humans ; Neoplasm Metastasis ; Osteosarcoma/metabolism ; Zinc Finger E-box-Binding Homeobox 1/genetics
References:
J Clin Oncol. 2015 Sep 20;33(27):3029-35. (PMID: 26304877)
Genome Res. 2009 Jan;19(1):92-105. (PMID: 18955434)
Ann Oncol. 2010 Oct;21 Suppl 7:vii320-5. (PMID: 20943636)
Cancer Res. 2012 Apr 1;72(7):1865-77. (PMID: 22350417)
Exp Ther Med. 2019 Mar;17(3):2235-2241. (PMID: 30783484)
Mol Med Rep. 2017 Sep;16(3):3606-3612. (PMID: 28714003)
Cell. 2018 Mar 22;173(1):20-51. (PMID: 29570994)
Sci Total Environ. 2017 Jun 1;587-588:305-315. (PMID: 28249752)
Mol Oncol. 2017 Jan;11(1):28-39. (PMID: 28085222)
Mol Oncol. 2017 Jul;11(7):792-804. (PMID: 28649800)
Int J Biochem Cell Biol. 2007;39(12):2153-60. (PMID: 17825600)
J Surg Oncol. 2012 Jun 15;105(8):830-4. (PMID: 22213004)
EXCLI J. 2017 May 05;16:618-627. (PMID: 28694763)
Curr Opin Pharmacol. 2014 Jun;16:15-23. (PMID: 24632219)
Expert Rev Anticancer Ther. 2016 May;16(5):543-56. (PMID: 26999418)
Sci Rep. 2017 Jul 14;7(1):5382. (PMID: 28710380)
J Cell Biochem. 2017 Nov;118(11):3765-3774. (PMID: 28379605)
PLoS One. 2012;7(3):e33663. (PMID: 22479426)
Cancer Res. 2011 Oct 1;71(19):6208-19. (PMID: 21852381)
ACS Med Chem Lett. 2013 Dec 10;5(2):143-8. (PMID: 24900787)
Trends Mol Med. 2014 Aug;20(8):460-9. (PMID: 25027972)
J Biochem Mol Biol. 2003 Jan 31;36(1):128-37. (PMID: 12542983)
Pathol Oncol Res. 2019 Jan;25(1):377-389. (PMID: 29138985)
Nat Commun. 2015 Jan 08;6:5917. (PMID: 25569036)
Stem Cells. 2018 Oct;36(10):1487-1500. (PMID: 30001480)
Cancer Cell. 2013 Jan 14;23(1):63-76. (PMID: 23328481)
Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9667-72. (PMID: 17535905)
Cell Mol Life Sci. 2009 Mar;66(5):773-87. (PMID: 19011757)
J Cancer Res Clin Oncol. 2013 Jul;139(7):1189-98. (PMID: 23568547)
Cell. 2009 Nov 25;139(5):871-90. (PMID: 19945376)
Nat Rev Genet. 2008 Feb;9(2):102-14. (PMID: 18197166)
Eur J Cancer. 2019 Mar;109:36-50. (PMID: 30685685)
Nature. 2004 Sep 16;431(7006):350-5. (PMID: 15372042)
Anticancer Drugs. 2018 Jun;29(5):440-448. (PMID: 29494357)
Pathol Oncol Res. 2019 Jan;25(1):11-20. (PMID: 28944406)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Carcinogenesis. 2019 Apr 29;40(2):335-348. (PMID: 30726934)
Biomed Pharmacother. 2019 Feb;110:400-408. (PMID: 30530042)
Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438)
Oncol Lett. 2014 Oct;8(4):1768-1774. (PMID: 25202407)
Contributed Indexing:
Keywords: microRNA-708-5p; osteosarcoma; epithelial-to-mesenchymal transition; metastasis
Substance Nomenclature:
0 (MIRN708 microRNA, human)
0 (MicroRNAs)
0 (ZEB1 protein, human)
0 (Zinc Finger E-box-Binding Homeobox 1)
Entry Date(s):
Date Created: 20200103 Date Completed: 20200821 Latest Revision: 20211112
Update Code:
20240104
PubMed Central ID:
PMC6967104
DOI:
10.3892/or.2019.7452
PMID:
31894343
Czasopismo naukowe
MicroRNA‑708‑5p (miR‑708‑5p) and epithelial‑​to‑mesenchymal transition (EMT) have been widely identified to contribute to the pathogenesis and progression of multiple cancers. However, the connection between miR‑708‑5p and EMT has not been sufficiently clarified. Therefore, our research aimed to investigate the impact of miR‑708‑5p on EMT and the metastasis of osteosarcoma (OS). We first analyzed the differentially expressed microRNAs (DEmiRNAs) from the GSE70367 dataset. We found that the expression of miR‑708‑5p was lower in OS cells. Overexpression of miR‑708‑5p was able to impair the migration and invasion of OS cells. Moreover, miR‑708‑5p inhibited EMT of OS cells MG63 and SaOS‑2, wherein E‑cadherin was increased, and N‑cadherin, vimentin, and Snail were decreased. Semaphorin 4C (SEMA4C), mitogen‑activated protein kinase kinase kinase 3 (MAP3K3), and zinc finger E‑box‑binding homeobox 1 (ZEB1) were predicted as target genes of miR‑708‑5p by bioinformatics method. Only ZEB1, one of the EMT‑inducing transcription factors, was validated as the direct target gene of miR‑708‑5p in OS cells through dual‑luciferase reporter assay and Western blot analysis. Knockdown of ZEB1 was found to inhibit the metastasis of MG63 and SaOS‑2 cells, whereas ZEB1 over-expression promoted their metastasis. In summary, miR‑708‑5p impaired the metastasis and EMT of OS, which was found to be mediated by inhibition of ZEB1.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies