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Tytuł:
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Investigation on glucocorticoid receptors within platelets from adult patients with immune thrombocytopenia.
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Autorzy:
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Yung KC; Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Xu CW; Hemodialysis Room, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Zhang ZW; Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Yu WJ; Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Li Q; Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Xu XR; Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Han YF; Department of Spine Surgery, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Wang XJ; Department of Spine Surgery, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
Yin J; Division of Hematology, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.; Department of Clinical Laboratory Medicine, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.; Translational Medicine Research Center, the Second Affiliated Hospital of Shantou University Medical College, Shantou, People's Republic of China.
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Źródło:
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Hematology (Amsterdam, Netherlands) [Hematology] 2020 Dec; Vol. 25 (1), pp. 37-42.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2016- : Abingdon : Taylor & Francis
Original Publication: [Amsterdam] : Newark, NJ : Harwood Academic Publishers ; International Publishers Distributor,
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MeSH Terms:
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Blood Platelets/*pathology
Purpura, Thrombocytopenic, Idiopathic/*pathology
Receptors, Glucocorticoid/*analysis
Adult ; Aged ; Blood Platelets/metabolism ; Female ; Flow Cytometry ; Humans ; Male ; Middle Aged ; Purpura, Thrombocytopenic, Idiopathic/genetics ; Receptors, Glucocorticoid/genetics ; Up-Regulation ; Young Adult
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Contributed Indexing:
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Keywords: Glucocorticoids (GC); Newly diagnosed Immune thrombocytopenia (ITP); enzyme linked immunosorbent assay (ELISA); flow cytometry; glucocorticoid receptor; nongenomic processes; platelets; reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
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Substance Nomenclature:
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0 (Receptors, Glucocorticoid)
0 (glucocorticoid receptor alpha)
0 (glucocorticoid receptor beta)
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Entry Date(s):
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Date Created: 20200107 Date Completed: 20200908 Latest Revision: 20200908
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Update Code:
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20240105
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DOI:
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10.1080/16078454.2019.1710025
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PMID:
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31905108
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Objective: The expression of glucocorticoid receptors within platelets from newly diagnosed Immune Thrombocytopenia (ITP) patients in the adult was investigated. Methods: GR expression in platelets from newly diagnosed ITP patients and healthy controls was measured using flow cytometry. Subsequently, platelets RNA and proteins were isolated and used for confirming the flow cytometry results by using RT-qPCR and ELISA. Results: Flow cytometry showed that the percentages of platelets expressing GRα and GRβ from ITP patients were significantly higher than those from healthy controls ( P < 0.05). qPCR and ELISA confirmed that GRα and GRβ were increased at both RNA transcription and protein expression levels within platelets from ITP patients compared with healthy controls. Conclusion: We speculated that the up-regulation of glucocorticoid receptor within platelets may be an important biological feature of platelets in patients with ITP, and may also play an important role in the treatment of ITP, which is worthy of further study.
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