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Tytuł:
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An Optimized Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry Assay for the Determination of Ethyl Palmitate in Hair.
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Autorzy:
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Bastiani MF; Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.; National Institute of Forensic Science and Technology (INCT Forense), Porto Alegre, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Lizot LLF; Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.; National Institute of Forensic Science and Technology (INCT Forense), Porto Alegre, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Da Silva ACC; Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Hahn RZ; Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.
Dries SS; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Perassolo MS; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Antunes MV; Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Linden R; Laboratory of Analytical Toxicology, Feevale University, Novo Hamburgo, Brazil.; National Institute of Forensic Science and Technology (INCT Forense), Porto Alegre, Brazil.; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, Novo Hamburgo, RS, Brazil.
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Źródło:
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Journal of analytical toxicology [J Anal Toxicol] 2020 May 18; Vol. 44 (4), pp. 402-409.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2012- : Oxford : Oxford University Press
Original Publication: 1977-<2011>: Niles, Ill., Preston Publications.
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MeSH Terms:
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Hair/*chemistry
Palmitic Acids/*analysis
Solid Phase Microextraction/*methods
Esters ; Fatty Acids ; Gas Chromatography-Mass Spectrometry ; Humans ; Sensitivity and Specificity
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Substance Nomenclature:
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0 (Esters)
0 (Fatty Acids)
0 (Palmitic Acids)
IRD3M534ZM (ethyl palmitate)
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Entry Date(s):
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Date Created: 20200108 Date Completed: 20200916 Latest Revision: 20200916
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Update Code:
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20240105
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DOI:
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10.1093/jat/bkz085
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PMID:
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31909801
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The use of hair as a matrix for the evaluation of chronic ethanol drinking behavior presents the advantage of a longer window of detection and higher specificity when compared to classical biochemical markers. The most recent recommendations the Society of Hair Testing (SOHT) indicate that ethyl palmitate (EtP) hair levels can be used to estimate the ethanol drinking behavior, alternatively to the combined measurement of four main fatty acid ethyl esters. In this study, solid-phase microextraction (SPME) conditions for the extraction of EtP from hair were optimized using response surface analysis, after a Box-Behnken experiment. Analyses were performed by GC-MS. The optimized HS-SPME conditions, using a PDMS-DVB (65 μm) fiber, were pre-adsorption time of 6 min, extraction time of 60 min and incubation temperature of 94°C. The linear range was 0.05 to 3 ng mg-1, with accuracy within 95.15-109.91%. Between-assay and within-assay precision were 8.58-12.53 and 6.12-6.82%, respectively. The extraction yield was 61.3-71.9%. The assay was applied to hair specimens obtained from 46 volunteers, all presenting EtP levels within the linear range of the assay. Using a statistically designed experiment, a sensitive SPME-GC-MS assay for the measurement of EtP in hair was developed and validated, requiring only 20 mg of hair.
(© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
