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Tytuł:
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Skeletal muscle cells derived from mouse skin cultures.
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Autorzy:
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Ohnota H; Department of Drug Discovery Science, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan; Research Division, R&D, Kissei Pharmaceutical Co. Ltd., 4365-1 Kashiwabara, Hotaka, Azumino, Nagano, 399-8304, Japan. Electronic address: .
Nakazawa H; Department of Drug Discovery Science, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.
Hayashi M; Safety Research Laboratory, R&D, Kissei Pharmaceutical Co. Ltd., 2320-1 Maki, Hotaka, Azumino, Nagano, 399-8304, Japan.
Okuhara Y; Safety Research Laboratory, R&D, Kissei Pharmaceutical Co. Ltd., 2320-1 Maki, Hotaka, Azumino, Nagano, 399-8304, Japan.
Honda T; Department of Drug Discovery Science, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.
d'Azzo A; Department of Genetics, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
Sekijima Y; Department of Medicine (Neurology and Rheumatology), Shinshu University School of Medicine, Matsumoto, 390-8621, Japan.
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Źródło:
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Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Jul 23; Vol. 528 (2), pp. 398-403. Date of Electronic Publication: 2020 Jan 08.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
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MeSH Terms:
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Tissue Culture Techniques*
Muscle Cells/*cytology
Muscle, Skeletal/*cytology
Skin/*cytology
Animals ; Cell Movement ; Disease Models, Animal ; Female ; Fibroblasts/cytology ; Male ; Mice ; Models, Biological ; Mucolipidoses/pathology ; Muscle Development ; Muscle Fibers, Skeletal/cytology ; Muscle Fibers, Skeletal/ultrastructure ; Neuraminidase/metabolism
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Contributed Indexing:
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Keywords: Myotube; PAX7; Panniculus carnosus; Sialidosis; Skin; Twitching
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Substance Nomenclature:
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EC 3.2.1.18 (Neuraminidase)
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Entry Date(s):
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Date Created: 20200114 Date Completed: 20210113 Latest Revision: 20210113
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Update Code:
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20240105
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DOI:
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10.1016/j.bbrc.2019.12.067
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PMID:
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31926596
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We have established a novel, simple, and highly reproducible method to generate skeletal muscle cells from mouse skin. Small pieces of skin from the back of mice were cultured in extracellular material-coated dishes in typical culture medium for about 3 weeks. Myotubes formed after about a week, grew into twitching myotubes, and became twitching myotube clumps after 3 weeks. Skeletal muscle cells are formed spontaneously with no induction. Myotubes were immunologically positive for myosin heavy chains, MyoD, and myogenin. Ultrastructural analysis revealed the presence of the sarcomere structure. Furthermore, PAX7 + /MyoD - muscle stem cells proliferated around these myotubes, and MyoD + /myogenin + /MHC -- cells were also observed. Moreover, we investigated the formation of skeletal muscle cells from the sialidosis mouse skin, and showed that it is decreased compared to that of the wild type. Our method to generate skeletal muscle cells from skin is thought to be useful for the investigation of muscle cell development and muscle-related disorders.
Competing Interests: Declaration of competing interest (1) This work was supported by a grant of Kissei Pharmaceutical CO., LTD. (2) Hideki Ohnota, Morimichi Hayashi, and Yuji Okuhara receive their salaries from Kissei Pharmaceutical CO., LTD. (3) A patent filing on this work is scheduled on December 4. (4) No other author has reported a potential conflict of interest relevant to this article.
(Copyright © 2020 Elsevier Inc. All rights reserved.)