Mycobacterium abscessus subsp. massiliense expressing bacterioferritin have improved resistance to stressful conditions.

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Tytuł:: Mycobacterium abscessus subsp. massiliense expressing bacterioferritin have improved resistance to stressful conditions.
Autorzy:: Oliveira FM; Department of Biosciences and Technology, Tropical Institute of Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
Marinho FV; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Oliveira SC; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Resende DP; Department of Biosciences and Technology, Tropical Institute of Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
Junqueira-Kipnis AP; Department of Biosciences and Technology, Tropical Institute of Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
Kipnis A; Department of Biosciences and Technology, Tropical Institute of Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
Źródło:: Journal of applied microbiology [J Appl Microbiol] 2020 Jun; Vol. 128 (6), pp. 1802-1813. Date of Electronic Publication: 2020 Feb 05.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2022- : Oxford : Oxford University Press
Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
MeSH Terms:: Bacterial Proteins/*metabolism
Cytochrome b Group/*metabolism
Ferritins/*metabolism
Mycobacterium abscessus/*pathogenicity
Mycobacterium abscessus/*physiology
Animals ; Bacterial Load ; Bacterial Proteins/genetics ; Cytochrome b Group/genetics ; Ferritins/genetics ; Mice ; Mycobacterium Infections, Nontuberculous/microbiology ; Mycobacterium Infections, Nontuberculous/pathology ; Mycobacterium abscessus/genetics ; Mycobacterium abscessus/growth & development ; Mycobacterium tuberculosis/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Stress, Physiological ; Virulence
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Grant Information:: 2017/24832-6 Fundação de Amparo à Pesquisa do Estado de São Paulo; 302660/2015-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 302974/2017-2 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 88887.364940/2019-00 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Universidade Federal de Goiás
Contributed Indexing:: Keywords: bacterioferritin; ferritin; iron homeostasis; nutritional immunity; pathogenicity; virulence
Substance Nomenclature:: 0 (Bacterial Proteins)
0 (Cytochrome b Group)
0 (Recombinant Proteins)
9007-73-2 (Ferritins)
9035-38-5 (bacterioferritin)
Entry Date(s):: Date Created: 20200125 Date Completed: 20200821 Latest Revision: 20200821
Update Code:: 20240104
DOI:: 10.1111/jam.14585
PMID:: 31975455
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Aims: The importance of bacterioferritin in the virulence and pathogenicity of the genus Mycobacterium is still unclear. The aim of this study was to analyse if the expression of a recombinant bacterioferritin from M. tuberculosis (Mtb) by Mycma could improve the capacity of this bacillus to resist the host defence mechanisms.
Methods and Results: Recombinant Mycma, expressing bacterioferritin (Rv1876) from Mtb, was developed by transformation with pMIP12_Rv1876. To determine bacterioferritin influence on Mycma physiology and virulence, the mycobacteria growth was analysed in vitro and in vivo. It was observed that the expression of bacterioferritin improved the growth rate of recombinant Mycma_BfrA under iron excess and oxidative stress, as compared to the wild type. Furthermore, in the murine model of infection, it was observed that Mycma_BfrA-infected mice had higher bacillary load and a more pronounced lesion in the lungs when compared with the wild type.
Conclusion: This study showed that bacterioferritin confers additional resistance to stress conditions, resulting in increased pathogenicity of Mycma during mice infection.
Significance and Impact of the Study: This study provides new insights about the importance of bacterioferritin in the virulence and pathogenicity of the Mycobacterium genus.
(© 2020 The Society for Applied Microbiology.)

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