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Tytuł:
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Overexpression of Arabidopsis aspartic protease APA1 gene confers drought tolerance.
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Autorzy:
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Sebastián D; Biological Research Institute, National Council of Scientific and Technique Research (CONICET), University of Mar del Plata, Mar del Plata (UNMDP), Argentina.
Fernando FD; Biological Research Institute, National Council of Scientific and Technique Research (CONICET), University of Mar del Plata, Mar del Plata (UNMDP), Argentina.
Raúl DG; Biological Research Institute, National Council of Scientific and Technique Research (CONICET), University of Mar del Plata, Mar del Plata (UNMDP), Argentina.
Gabriela GM; Biological Research Institute, National Council of Scientific and Technique Research (CONICET), University of Mar del Plata, Mar del Plata (UNMDP), Argentina. Electronic address: .
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Źródło:
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Plant science : an international journal of experimental plant biology [Plant Sci] 2020 Mar; Vol. 292, pp. 110406. Date of Electronic Publication: 2020 Jan 07.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: <2010>- : Shannon, Co. Clare : Elsevier Ireland
Original Publication: Shannon [Clare] : Elsevier Scientific Publishers Ireland Ltd., c1985-
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MeSH Terms:
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Droughts*
Gene Expression Regulation, Plant*
Signal Transduction*
Abscisic Acid/*metabolism
Arabidopsis/*physiology
Arabidopsis Proteins/*genetics
Aspartic Acid Endopeptidases/*genetics
Acclimatization ; Arabidopsis/genetics ; Arabidopsis Proteins/metabolism ; Aspartic Acid Endopeptidases/metabolism
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Contributed Indexing:
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Keywords: ABA signaling; Arabidopsis; Aspartic protease; Drought tolerance; Guard cell
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Substance Nomenclature:
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0 (Arabidopsis Proteins)
72S9A8J5GW (Abscisic Acid)
EC 3.4.23.- (AT1G11910 protein, Arabidopsis)
EC 3.4.23.- (Aspartic Acid Endopeptidases)
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Entry Date(s):
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Date Created: 20200202 Date Completed: 20200831 Latest Revision: 20200930
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Update Code:
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20240105
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DOI:
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10.1016/j.plantsci.2020.110406
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PMID:
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32005402
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Drought is an environmental stress that severely affects plant growth and crop production. Different studies have focused on drought responses but the molecular bases that regulate these mechanisms are still unclear. We report the participation of Aspartic Protease (APA1) in drought tolerance. Overexpressing APA1 Arabidopsis plants (OE-APA1), showed a phenotype more tolerant to drought compared with WT. On the contrary, apa1 insertional lines were more sensitive to this stress compared to WT plants. Morphological and physiological differences related with the water loss were observed between leaves of OE- APA1 and WT plants. OE-APA1 leaves showed lower stomata index and stomata density as well as a smaller of the stomatic aperture compared to WT plants. qPCR analysis in OE-APA1 leaves, showed higher expression levels of genes related to ABA signaling and synthesis. Analysis of plant lines expressing APA1 promoter fused to GUS showed that APA1 is expressed in epidermal and stomata cells. In summary, this work suggests that APA1 is involved in ABA-dependent response that its overexpression confers drought tolerance in Arabidopsis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
