Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays. - OpacWWW

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Tytuł:: Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays.
Autorzy:: Sun Z; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Sun Y; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Li Y; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Luan X; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Chen H; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Wu H; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Peng B; Department of Pathology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Lu C; Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Źródło:: Molecular medicine reports [Mol Med Rep] 2020 Mar; Vol. 21 (3), pp. 1572-1580. Date of Electronic Publication: 2020 Jan 16.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Athens, Greece : D. A. Spandidos
MeSH Terms:: Protein Interaction Mapping*/methods
Two-Hybrid System Techniques*
Bacterial Proteins/*metabolism
Carrier Proteins/*metabolism
Chlamydia trachomatis/*enzymology
Glycogen Synthase/*metabolism
Bacterial Proteins/genetics ; Chlamydia Infections/metabolism ; Chlamydia Infections/microbiology ; Glycogen Synthase/genetics ; HeLa Cells ; Host-Pathogen Interactions ; Humans ; Plasmids/genetics ; Prohibitins ; Protein Binding ; Reproducibility of Results
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Contributed Indexing:: Keywords: Chlamydia trachomatis; glycogen synthase; yeast two-hybrid assay
Substance Nomenclature:: 0 (Bacterial Proteins)
0 (Carrier Proteins)
0 (PHB protein, human)
0 (Prohibitins)
EC 2.4.1.11 (Glycogen Synthase)
Entry Date(s):: Date Created: 20200205 Date Completed: 20201021 Latest Revision: 20211204
Update Code:: 20240105
PubMed Central ID:: PMC7003024
DOI:: 10.3892/mmr.2020.10947
PMID:: 32016474
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Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (GlgA) is a chlamydial secretory protein, which localizes in the lumen of chlamydial inclusion bodies and the cytosol of host cells. In order to improve understanding of the roles of GlgA in chlamydial pathogenesis, four proteins that interact with GlgA, Homo sapiens CXXC finger protein 1, prohibitin (PHB), gelsolin‑like actin‑capping protein and apolipoprotein A‑I binding protein were identified using yeast two‑hybrid assays. The functions of these proteins are complex, and preliminary results suggested that PHB interacts with GlgA. However, further studies are required to determine the specific interactions of these proteins with GlgA. The findings of the present study may provide a direction and foundation for future studies focusing on the mechanism of GlgA in C. trachomatis infection.

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Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays.