Recombinant spider silk coatings functionalized with enzymes targeting bacteria and biofilms.

Szczegóły
Abstrakt

Tytuł:: Recombinant spider silk coatings functionalized with enzymes targeting bacteria and biofilms.
Autorzy:: Seijsing F; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
Nilebäck L; Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Öhman O; Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Pasupuleti R; Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Ståhl C; Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Seijsing J; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
Hedhammar M; Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Źródło:: MicrobiologyOpen [Microbiologyopen] 2020 Apr; Vol. 9 (4), pp. e993. Date of Electronic Publication: 2020 Feb 07.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Oxford : Wiley-Blackwell
MeSH Terms:: Anti-Bacterial Agents/*pharmacology
Bacteria/*drug effects
Bacterial Proteins/*pharmacology
Biofilms/*drug effects
Glycoside Hydrolases/*pharmacology
Silk/*pharmacology
Bacteria/growth & development ; Bacterial Adhesion/drug effects ; Bacterial Proteins/genetics ; Bacteriophages/metabolism ; Biofilms/growth & development ; Glycoside Hydrolases/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/pharmacology ; Silk/genetics ; Viral Proteins/genetics
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Contributed Indexing:: Keywords: antibacterial; antibiofilm; coating; endolysin; recombinant spider silk
Substance Nomenclature:: 0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
0 (Recombinant Proteins)
0 (Silk)
0 (Viral Proteins)
EC 3.2.1.- (Glycoside Hydrolases)
EC 3.2.1.- (dispersin B, Actinobacillus actinomycetemcomitans)
Entry Date(s):: Date Created: 20200208 Date Completed: 20210125 Latest Revision: 20240328
Update Code:: 20240329
PubMed Central ID:: PMC7142364
DOI:: 10.1002/mbo3.993
PMID:: 32032479
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Bacteria forming biofilms on surgical implants is a problem that might be alleviated by the use of antibacterial coatings. In this article, recombinant spider silk was functionalized with the peptidoglycan degrading endolysin SAL-1 from the staphylococcal bacteriophage SAP-1 and the biofilm-matrix-degrading enzyme Dispersin B from Aggregatibacter actinomycetemcomitans using direct genetic fusion and/or covalent protein-protein fusion catalyzed by Sortase A. Spider silk assembly and enzyme immobilization was monitored using quartz crystal microbalance analysis. Enzyme activity was investigated both with a biochemical assay using cleavage of fluorescent substrate analogues and bacterial assays for biofilm degradation and turbidity reduction. Spider silk coatings functionalized with SAL-1 and Disperin B were found to exhibit bacteriolytic effect and inhibit biofilm formation, respectively. The strategy to immobilize antibacterial enzymes to spider silk presented herein show potential to be used as surface coatings of surgical implants and other medical equipment to avoid bacterial colonization.
(© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)

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