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Tytuł pozycji:

LC-MS/MS method for the determination of hormones: Validation, application and health risk assessment in various bovine matrices.

Tytuł:
LC-MS/MS method for the determination of hormones: Validation, application and health risk assessment in various bovine matrices.
Autorzy:
Moussa F; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy; Doctoral School of Science and Technology Lebanese University, Beirut, Lebanon.
Mokh S; National Council for Scientific Research (CNRS), Lebanese Atomic Energy Commission (LAEC), Laboratory for Analysis of Organic Compound (LACO), Beirut, Lebanon.
Doumiati S; Faculty of Public Health I - Lebanese University, Hadath, Lebanon.
Barboni B; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy.
Bernabò N; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy.
Al Iskandarani M; National Council for Scientific Research (CNRS), Lebanese Atomic Energy Commission (LAEC), Laboratory for Analysis of Organic Compound (LACO), Beirut, Lebanon; Faculty of Public Health I - Lebanese University, Hadath, Lebanon. Electronic address: .
Źródło:
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2020 Apr; Vol. 138, pp. 111204. Date of Electronic Publication: 2020 Feb 17.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Exeter : Elsevier Science Ltd
Original Publication: Oxford ; New York : Pergamon Press, c1982-
MeSH Terms:
Chromatography, High Pressure Liquid*
Tandem Mass Spectrometry*
Animals ; Bile/chemistry ; Cattle ; Hair/chemistry ; Kidney/chemistry ; Limit of Detection ; Liver/chemistry ; Muscles/chemistry ; Progesterone/analysis ; Reproducibility of Results ; Risk Assessment ; Sensitivity and Specificity ; Testosterone/analysis
Contributed Indexing:
Keywords: Animal matrices; Hormones; Liquid chromatography tandem mass spectrometry; Matrix effect; QusEChERS; Validation
Substance Nomenclature:
3XMK78S47O (Testosterone)
4G7DS2Q64Y (Progesterone)
Entry Date(s):
Date Created: 20200222 Date Completed: 20201130 Latest Revision: 20201130
Update Code:
20240105
DOI:
10.1016/j.fct.2020.111204
PMID:
32081729
Czasopismo naukowe
A new method, using liquid chromatography coupled to mass spectrometry (LC-MS/MS) for the detection of fourteen natural and synthetic hormones in muscles, was validated in other bovine matrices (liver, kidney, bile and hair) according to the Decision Commission 2002/657/EC. As result, this method demonstrates good linearity (R 2  > 0.99) as well as accuracy with coefficients of variation for repeatability and reproducibility lower than 23%. Moreover, the values of decision limit (CCα) and detection capability (CCβ) were determined indicating values ranging from 0.13 to 0.86 μg/kg and 0.25-1.72 μg/k for the majority of analytes. Recovery rate in the different matrices varied from 51.5 to 107%. Indeed, this method has been successfully applied to detect anabolic hormones in eighty-eight samples (muscle, liver, kidney, and bile) collected from different local slaughterhouses. Results showed that progesterone was found in 30 samples at concentrations ranging from 0.11 to 11.7 μg/kg, while testosterone was detected in 34 samples at concentrations ranging from 0.5 to 9.52 μg/kg. All bile samples contain epi-testosterone at concentration ranging from 0.89 to 280 μg/kg. These obtained data were used to calculate the estimated daily intake, hazard quotient and hazard index as exposure assessment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)

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