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Tytuł:
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Three-dimensional visualization of coronary microvasculature in rats with myocardial infarction.
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Autorzy:
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Liu X; Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430033, China.
Wang Y; Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430033, China.
Tang M; College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Liu Y; Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430033, China.
Hu L; Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430033, China.
Gu Y; Department of Cardiology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430033, China. Electronic address: .
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Źródło:
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Microvascular research [Microvasc Res] 2020 Jul; Vol. 130, pp. 103990. Date of Electronic Publication: 2020 Feb 20.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
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Język:
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English
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Imprint Name(s):
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Original Publication: New York, Academic Press.
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MeSH Terms:
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Imaging, Three-Dimensional*
Microscopy, Confocal*
Microscopy, Fluorescence*
Coronary Vessels/*diagnostic imaging
Microvessels/*diagnostic imaging
Myocardial Infarction/*diagnostic imaging
Animals ; Carbocyanines/administration & dosage ; Disease Models, Animal ; Feasibility Studies ; Fluorescent Dyes/administration & dosage ; Predictive Value of Tests ; Rats, Sprague-Dawley
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Contributed Indexing:
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Keywords: DiI; Laser scanning confocal microscope; Microvascular 3D visualization technology
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Substance Nomenclature:
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0 (Carbocyanines)
0 (Fluorescent Dyes)
40957-95-7 (3,3'-dioctadecylindocarbocyanine)
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Entry Date(s):
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Date Created: 20200224 Date Completed: 20200923 Latest Revision: 20200923
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Update Code:
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20240105
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DOI:
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10.1016/j.mvr.2020.103990
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PMID:
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32088162
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Background: Assessment of the coronary microcirculation remains challenging.
Objective: we explored the feasibility of evaluating the coronary microvasculature in rats with myocardial infarction (MI) using a three-dimensional visualization technique.
Methods: Animals were divided into the sham operation group (S), MI 45 min group (M45), and MI 180 min group (M180). Opened microvessels were labelled with the fluorescent dye DiI (1, 1'-dioctadecyl-3, 3, 3'3'-tetramethylindo carbocyanine perchlorate) using a heart perfusion method. The microvascular distribution and opening status were observed under laser scanning confocal microscopy, which was adjusted to facilitate evaluation of subjects around 6 to 20 μm.
Results: Microvascular vessels (6-20 μm) were successfully labelled by DiI. Intact and clear three-dimensional microvascular structures were observed in myocardium of sham rats and remote non-infarct myocardial tissue of MI rats, while there was almost no microvascular structure in the infarct area of the M45 group, and only a small amount of microvascular visualization was visualized in the infarct area of the M180 group. The microvascular area and microvascular density in M45 group and M180 group in the infarct border zone were significantly lower than corresponding area in S group.
Conclusion: Three-dimensional visualization of opened coronary microvascular vessels is feasible in DiI-labelled myocardium in this rat MI model. This novel technique might be useful for defining the underlying mechanisms of coronary microvascular diseases and observe the efficacy of various therapy strategies on coronary microvessels.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
