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Tytuł:
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Poly-functional T helper cells in human tonsillar mononuclear cells.
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Autorzy:
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Yu S; From the Institute of Immunology, Zhongshan School of Medicine; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
Luo X; From the department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou, China.
Yang B; From the Institute of Immunology, Zhongshan School of Medicine; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
Xiao L; From the Institute of Immunology, Zhongshan School of Medicine; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
Wu X; From the Allergy Center, Otohinolaryngology Hospital, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Li H; From the Allergy Center, Otohinolaryngology Hospital, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Wu C; From the Institute of Immunology, Zhongshan School of Medicine; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
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Źródło:
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European cytokine network [Eur Cytokine Netw] 2019 Dec 01; Vol. 30 (4), pp. 114-122.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Montrouge, France : John Libbey Eurotext Ltd.
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MeSH Terms:
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Gene Expression/*immunology
Palatine Tonsil/*immunology
T-Lymphocytes, Helper-Inducer/*immunology
Adolescent ; Adult ; B-Lymphocytes/cytology ; B-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/cytology ; CD8-Positive T-Lymphocytes/immunology ; Female ; Flow Cytometry ; Humans ; Interferon-gamma/genetics ; Interferon-gamma/immunology ; Interleukin-17/genetics ; Interleukin-17/immunology ; Interleukin-2/genetics ; Interleukin-2/immunology ; Interleukins/genetics ; Interleukins/immunology ; Ionomycin/pharmacology ; Lymphocyte Activation ; Male ; Palatine Tonsil/cytology ; Primary Cell Culture ; Single-Cell Analysis ; T-Lymphocytes, Helper-Inducer/cytology ; T-Lymphocytes, Helper-Inducer/drug effects ; Tetradecanoylphorbol Acetate/pharmacology ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/immunology ; Interleukin-22
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Contributed Indexing:
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Keywords: cytokines; poly-functional Th cells; tonsils
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Substance Nomenclature:
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0 (Interleukin-17)
0 (Interleukin-2)
0 (Interleukins)
0 (Tumor Necrosis Factor-alpha)
56092-81-0 (Ionomycin)
82115-62-6 (Interferon-gamma)
MKM3CA6LT1 (interleukin-21)
NI40JAQ945 (Tetradecanoylphorbol Acetate)
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Entry Date(s):
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Date Created: 20200226 Date Completed: 20200521 Latest Revision: 20231213
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Update Code:
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20240105
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DOI:
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10.1684/ecn.2015.0368
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PMID:
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32096472
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Tonsils are important lymphoid organs in which B cells and T cells complete their maturation and identify cells that are infected by pathogens. However, the functions of T cells in human tonsils remain unclear, especially the characteristics of polyfunctional CD4 + T helper cells. In this study, we used multi-color flow cytometry to analyze the expression or co-expression of effector cytokines in CD4 + T cells from tonsillar tissues. We have demonstrated that tonsillar CD4 + T cell can express various Th effector cytokines after short-term polyclonal stimulation, and that cytokine-producing CD4 + T cells were CD45RO + T cells. In addition, we analyzed the co-expression of two or more kinds of cytokines at the level of a single cell. The results showed that tonsillar CD4 + T cells exhibited polyfunctionality by co-expressing two to five kinds of cytokines in the same time. These data furnished a basic theory for further understanding the differentiation of polyfunctional Th cells in human tonsils and their functions in resisting invasive microorganisms.
