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Tytuł:
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Enterococcus faecium L-15 Extract Enhances the Self-Renewal and Proliferation of Mouse Skin-Derived Precursor Cells.
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Autorzy:
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Kim K; Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul, 08826, South Korea.
Park S; Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul, 08826, South Korea.; Biomedical Research Institute, Neoregen Biotech Co., Ltd., Gyeonggi-do, 16614, South Korea.
Kim H; Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul, 08826, South Korea.
Min S; Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul, 08826, South Korea.
Ku S; Fermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN, 37132, USA.
Seo J; Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul, 08826, South Korea. .; Biomedical Research Institute, Neoregen Biotech Co., Ltd., Gyeonggi-do, 16614, South Korea. .
Roh S; Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul, 08826, South Korea. .
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Źródło:
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Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2020 Dec; Vol. 12 (4), pp. 1492-1501.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: New York, NY. : Springer
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MeSH Terms:
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Cell Lineage/*drug effects
Complex Mixtures/*pharmacology
Enterococcus faecium/*chemistry
Gene Expression Regulation/*drug effects
Probiotics/*pharmacology
Stem Cells/*drug effects
Animals ; Cell Cycle/drug effects ; Cell Cycle/genetics ; Cell Differentiation/drug effects ; Cell Lineage/genetics ; Cell Proliferation/drug effects ; Complex Mixtures/chemistry ; Embryo, Mammalian ; Enterococcus faecium/metabolism ; Epithelial Cells/cytology ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Glycogen Synthase Kinase 3 beta/genetics ; Glycogen Synthase Kinase 3 beta/metabolism ; Kruppel-Like Factor 4 ; Kruppel-Like Transcription Factors/genetics ; Kruppel-Like Transcription Factors/metabolism ; Mice ; Mice, Inbred ICR ; Octamer Transcription Factor-3/genetics ; Octamer Transcription Factor-3/metabolism ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Primary Cell Culture ; Probiotics/chemistry ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; SOXB1 Transcription Factors/genetics ; SOXB1 Transcription Factors/metabolism ; Signal Transduction ; Stem Cells/cytology ; Stem Cells/metabolism
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Contributed Indexing:
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Keywords: Lactic acid bacteria; PI3K/Akt signaling pathway; Proliferation; Self-renewal; Skin-derived precursor cell (SKP)
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Substance Nomenclature:
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0 (Complex Mixtures)
0 (Kruppel-Like Factor 4)
0 (Kruppel-Like Transcription Factors)
0 (Octamer Transcription Factor-3)
0 (Pou5f1 protein, mouse)
0 (SOXB1 Transcription Factors)
0 (Sox2 protein, mouse)
EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
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Entry Date(s):
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Date Created: 20200313 Date Completed: 20210611 Latest Revision: 20211204
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Update Code:
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20240105
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DOI:
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10.1007/s12602-020-09635-w
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PMID:
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32162154
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Lactic acid bacteria (LAB) in the gastrointestinal tract have beneficial health effects. LAB activate the proliferation of intestinal stem cells and speed the recovery of damaged intestinal cells, but little is known about effect of LAB on other adult stem cells. In this study, a cell-free extract of Enterococcus faecium L-15 (L15) was exposed to mouse skin-derived precursor cells (SKPs), and the changes in characteristics associated with proliferation and self-renewal capacity were investigated. L15 increased the size of the spheres and the proliferation rate of SKPs. Cell cycle analysis revealed that cells in the S-phase increased after treatment with L15. In the L15-treated group, the total number of spheres significantly increased. The expression level of pluripotency marker genes also increased, while the mesenchymal lineage-related differentiation marker genes significantly decreased in the L15-treated group. The PI3K/Akt signaling pathway was activated by L15 in SKPs. These results indicate that L15 enhances proliferation and self-renewal of SKPs and may be used as a supplement for stem cell maintenance or application of stem cell therapy. This is the first report to investigate the functional effects of E. faecium on the proliferation and self-renewal capacity of SKPs.
